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Enzymology of intracellular membrane fusion

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Summary

A combination of cell-free biochemical and morphological studies has revealed that a coated bud-coated vesicle transport system shuttles newly synthesized proteins through the successive processing compartments of the Golgi apparatus. These “Golgi coated vesicles” operate in a manner formally analogous to the clathrin coated pitcoated vesicle system responsible for receptor-mediated endocytosis; however Golgi coated vesicles do not contain clathrin.

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Abbreviations

ER:

endoplasmic reticulum

VSV:

vesicular stomatitis virus

TGN:

trans Golgi network

GCVs:

Golgiderived coated vesicule

NEM:

N-ethylmaleimide

NSF:

NEM-sensitive fusion protein

SNAP:

soluble NSF-attachment protein(s)

ARF:

ADP-ribosylation factor

AlF4 :

aluminumfluoride

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Authors and Affiliations

  1. Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA

    J. E. Rothman

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  1. J. E. Rothman
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Rothman, J.E. Enzymology of intracellular membrane fusion. Klin Wochenschr 69, 98–104 (1991). https://doi.org/10.1007/BF01795952

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  • DOI: https://doi.org/10.1007/BF01795952

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