Summary
The dimeric enzyme,α-Glycerophosphate dehydrogenase, was purified from eight Drosophila species by the method of Collier et al. (1976). The enzymes were inactivated at high pH and the conditions sufficient for reactivation were established. Electrophoretic patterns of reactivatedα-glycerophosphate dehydrogenases which were mixed following inactivation of two species' enzymes, demonstrate that high pH dissociates the enzyme into its constituent subunits and reactivation involves subunit reassociation. Twenty interspecific combinations of dissociated enzymes were allowed to reassociate, and the amounts of both heterospecific and homospecific enzyme activity and protein were determined by densitometry. In all 20 tests there were no differences between observed and expected heterospecific:homospecific enzyme ratios. These results are consistent with the very slow rate of evolution of this enzyme in the family Drosophilidae (Collier and MacIntyre, 1977).
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Collier, G.E., MacIntyre, R.J. Quantitative subunit hybridization of drosophilaα-Glycerophosphate dehydrogenase. J Mol Evol 12, 173–182 (1978). https://doi.org/10.1007/BF01733265
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DOI: https://doi.org/10.1007/BF01733265