Summary
A highly sensitive radioimmunoassay to measure atrial natriuretic peptide (ANP) concentration in urine has been established, and its clinical usefulness is presented. ANP in urine was stable at 4° C for several days and was easily measured by our radioimmunoassay. The average ANP excretion in 65 healthy persons was 25.0±1.4 ng/day (mean ± SEM) and the fractional excretion of ANP was 0.7±0.05%. In 14 patients with congestive heart failure, the average ANP excretion was 119.2±29.4 ng/day, which decreased to 53.3±11.0 after successful treatment.
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Abbreviations
- ANP:
-
atrial natriuretic peptide
- hANP:
-
human atrial natriuretic peptide
- RIA:
-
radioimmunoasay
- NSB:
-
non specific bound
- FEANP :
-
the fractional excretion of atrial natriuretic peptide
- FENa :
-
the fractional excretion of sodium
- SIADH:
-
the syngrome of inappropriate secretion of antidiuretic hormone
References
Cheung CY, Gibbs DM, Brace RA (1987) Atrial natriuretic factor in maternal and fetal sheep. Am J Physiol 252:E279-E282
Cody RJ, Atlas SA, Laragh JH, Kubo SH, Covit AB, Ryman KS, Shaknovich A, Pondolfino K, Clark M, Camargo MJF, Scarborough RM, Lewicki JA (1986) Atrial natriuretic factor in normal subjects and heart failure patients. Plasma levels and renal, hormonal, and hemodynamic responses to peptide infusion. J Clin Invest 78:1362–1374
Hartter E, Weissel M, Stummvoll HK, Woloszczuk W, Punzengruber C, Ludvik B (1985) Atrial natriuretic peptide concentrations in blood from right atrium in patient with severe right heart failure. Lancet ii:93–94
Huang CL, Ives HE, Cogan MG (1986) In vivo evidence that cGMP is the second messenger for atrial natriuretic factor. Proc Natl Acad Sci 83:8015–8018
Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of alpha-human atrial natriuretic polypeptide (α-hANP). Biochem Biophys Res Commun 118:131–139
Katoh Y, Kurosawa T, Takeda S, Sakamoto H, Marumo F, Kikawada R (1986) Atrial natriuretic peptide levels in treated congestive heart failure. Lancet i:851
Maack T, Marion DN, Camargo MJ, Kleinert HD, Laragh JH, Vaughan Jr ED, Atlas SA (1984) Effect of auriculin (atrial natriuretic factor) on blood pressure, renal function, and the renin-aldosterone system in dogs. Am J Med 77:1069–1075
Marumo F, Sakamoto H, Ando K, Ishigami T, Kawakami M (1986) A highly sensitive radioimmunoassay of atrial natriuretic peptide (ANP) in human plasma and urine. Biochem Biophys Res Commun 137:231–236
Nakao K, Sugawara A, Morii N, Sakamoto M, Yamada T, Itoh H, Shino S, Saito Y, Nishimura K, Ban T, Kangawa K, Matsuo H, Imura H (1986) The pharmacokinetics of α-human atrial natriuretic polypeptide in healthy subjects. Eur J Clin Pharmacol 31:101–103
Rasher W, Tulassay T, Lang RE (1985) Atrial natriuretic peptide in plasma of volume-overloaded children with chronic renal failure. Lancet ii:303–305
Sakamoto H, Inoue K, Marumo F (1986) High plasma atrial natriuretic peptide independent of sodium balance in SIADH. JAMA 256:1293–1294
Sugawara A, Nakao K, Morii Y, Sakamoto M, Suda M, Shimokura M, Kiso Y, Kihara M, Yamori Y, Nishimura K, Soneda J, Ban T, Imura H (1985) α-human atrial natriuretic polypeptide is released from the heart and circulates in the body. Biochem Biophys Res Commun 129:439–446
Yamaji T, Ishibashi M, Takaku F (1985) Atrial natriuretic factor in human blood. J Clin Invest 76:1705–1709
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Ando, K., Umetani, N., Kurosawa, T. et al. Atrial natriuretic peptide in human urine. Klin Wochenschr 66, 768–772 (1988). https://doi.org/10.1007/BF01726575
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DOI: https://doi.org/10.1007/BF01726575