Abstract
The nucleotide sequences of the neuraminidase (NA) genes of the A/Leningrad/134/57 (H2N2) wild-type (Len/wt) virus as well as two of its live attenuated, cold-adapted (ca) variants, A/Leningrad/134/17/57 (Len/17) and A/Leningrad/134/47/57 (Len/47), were determined. In comparison with Len/wt, one nucleotide change (C-225 to A) was found in the NA gene of Len/17. This change codes for a Thr-to-Asn substitution at position 69 of NA. The NA gene of the more attenuated Len/47 ca virus has one silent (T-814 to C) and two coding nucleotide substitutions, C-78 to T (Ala-20 to Val) and C-225 to A (Thr-69 to Asn). These sequence data were used to design a PCR-restriction technique to determine the origin of the NA gene in candidate live, attenuated vaccine reassortants made by reassorting these ca strains with current field viruses.
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References
Zhdanov V.M., in Kendal A. and Patriarca P. (eds).Options for the Control of Influenza: UCLA Symposia on Molecular and Cellular Biology No. 36, Alan R. Liss, New York, 1986, pp. 193–205.
Kendal A.P., Maassab H.F., Alexandrova G.I., and Ghendon Y.Z., Antiviral Res1 339–365, 1981.
Klimov A.I., Cox N.J., Yotov W.V., Rocha E., Alexandrova G.I., and Kendal A.P., Virology186 795–797, 1992.
Colman P.M., in Krug R.M. (ed).The Influenza Viruses. Plenum Press, New York, 1989, pp. 175–218.
Cox N.J., Kendal A.P., Shilov A.A., Alexandrova G.I., Ghendon Y.Z., and Klimov A.I., J Gen Virol66 1697–1704, 1985.
Medvedeva T.E., Kudriavtseva V.K., Zhikhareva I.V., Romashkina V.V., Alexandrova G.I., and Klimov A.I., Voprosy Virusologii36 96–100, 1991.
Klimov A.I. and Cox N.J., J Virol MethodsS2 41–49, 1995.
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The nucleotide sequence data reported in this paper have been submitted to the GenBank nucleotide sequence database and have been assigned accession numbers L37329-L37331.
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Klimov, A.I., Romanova, J.R., Egorov, A.Y. et al. Nucleotide sequences of the neuraminidase genes of influenza A/Leningrad/134/57 (H2N2) virus and two of its live, attenuated, cold-adapted variants. Virus Genes 10, 95–98 (1995). https://doi.org/10.1007/BF01724302
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DOI: https://doi.org/10.1007/BF01724302