Skip to main content
SpringerLink
Log in

Human colonic (Na++K+)-ATPase and specific ouabain binding are not influenced by lipoxygenase products or superoxide radicals

  • Wissenschaftliche Kurzmitteilung
  • Published:
Klinische Wochenschrift Aims and scope Submit manuscript

Summary

The effects of lipoxygenase products (5-, 12-, 15-HETE, LTB4) and superoxide radicals on human colonic (Na++K+)-ATPase and specific ouabain binding were measured. No significant inhibition in concentrations up to 3 × 10−5 M was observed. The results are discussed with regard to a possible role of lipoxygenase products and radicals in the pathogenesis of water and electrolyte disturbances in various diarrheal states including inflammatory bowel disease.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Abbreviations

IBD:

Inflammatory bowel disease

HETE:

Hydroxytetraenoic acid

LTB4 :

Leukotriene B4

References

  1. Allgayer H, Stenson WF (1988) A comparison of effects of sulfasalazine and its metabolites on the metabolism of endogenous vs exogenous arachidonic acid. Immunopharmacology (in press)

  2. Allgayer H, Brown L, Kruis W, Erdmann E, Paumgartner G (1986) Inhibition of human colonic (Na++K+)-ATPase by arachidonic and linoleic acid. Naunyn Schmiedeberg's Arch Pharmacol 332:398–402

    Article  CAS  Google Scholar 

  3. Allgayer H, Brown L, Kruis W, Erdmann E, Wiebecke B, Paumgartner G (1988) Inverse relationship between colonic (Na++K+)-ATPase and the degree of mucosal inflammation in inflammatory bowel disease. Dig Dis Sci (in press)

  4. Craven ZPA, Pfanstiel J, Saito R, DeRubertis FR (1987) Actions of sulfasalazine and 5-aminosalicylic acid as reactive oxygen ion scavengers in the suppression of bile acid induced increases in colonial epithelial cell loss and proliferative activity. Gastroenterology 92:1998–2008

    Article  CAS  PubMed  Google Scholar 

  5. Cross CE, Halliwell B, Borish ET, Pryer WA, et al. (1987) Davis Conference: Oxygen radicals and human disease. Ann Intern Med 107:526–545

    Article  CAS  PubMed  Google Scholar 

  6. Musch MW, Miller RJ, Field M, Siegel MJ (1982) Stimulation of colonic secretion by lipoxygenase metabolites of arachidonic acid. Science 217:1255–1256

    Article  CAS  PubMed  Google Scholar 

  7. Quigley JP, Gotterer GS (1973) Distribution of Na++K+)-stimulated ATPase activity in rat intestinal mucosa. Biochim Biophys Acta 173:456–468

    Article  Google Scholar 

  8. Rachmilewitz D, Treves AJ, Ligumski M, Sharon P, Zifroni A, Karmeli F (1984) Possible role of prostanoids as mediators in the pathogenesis of inflammatory bowel disease. In: Rachmilewitz D, Pena A (eds) Inflammatory bowel disease. Martinus Nihoff, The Hague Boston London, p 161

    Google Scholar 

  9. Sharon P, Stenson WF (1984) Enhanced synthesis of leukotriene B4 by colonic mucosa in inflammatory disease. Gastroenterology 86:453–457

    CAS  PubMed  Google Scholar 

  10. Sharon P, Karmeli F, Rachmilewitz D (1984) Effect of prostanoids on human intestinal (Na++K+)-ATPase activity. Isr J Med 20:677–682

    CAS  Google Scholar 

  11. Schoner W, Ilberg CV, Kramer R, Seubert W (1967) On the mechanisms of Na+ and K+ stimulated hydrolysis of adenosinetriphosphate. 1. Purification and properties of Na+ and K+ activated ATPase from ox brain. Eur J Biochem 1:334–343

    Article  CAS  PubMed  Google Scholar 

  12. Stirling CE (1972) Radioautographic localization of sodium pump sites in rabbit intestine. J Cell Biol 53:704–714

    Article  CAS  PubMed  PubMed Central  Google Scholar 

  13. Wanitschke R, Goerg KJ (1983) Intestinale Sekretion bei entzündlichen Darmerkrankungen. In: Ottenjann R, Fahrländer H (eds) Entzündliche Darmerkrankungen, Springer, Berlin Heidelberg New York Tokyo, p 48

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Medizinische Klinik II, Klinikum Großhadern, Universität München, Germany

    H. Allgayer & W. Kruis

  2. Medizinische Klinik I, Klinikum Großhadern, Universität München, Germany

    E. Erdmann

  3. Klinikum Karlsruhe, Moltkestr. 14, D-7500, Karlsruhe

    H. Allgayer

Authors
  1. H. Allgayer
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. Kruis
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. E. Erdmann
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Supported by DFG (Er 65/4-4)

Rights and permissions

Reprints and permissions

About this article

Check for updates. Verify currency and authenticity via CrossMark

Cite this article

Allgayer, H., Kruis, W. & Erdmann, E. Human colonic (Na++K+)-ATPase and specific ouabain binding are not influenced by lipoxygenase products or superoxide radicals. Klin Wochenschr 66, 599–600 (1988). https://doi.org/10.1007/BF01720836

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01720836

Key words

Search

Navigation