Summary
The nucleotide sequence of RNA-1 of an isolate of the H serotype of Indian peanut clump virus (IPCV-H) was shown to comprise 5 841 nucleotides. The RNA contains three open reading frames (ORF) which are between nucleotides 133 and 3 522, nucleotides 3 526 and 5 103 (assuming expression by suppression of the ORF 1 termination codon) and nucleotides 5 168 and 5 539. The encoded polypeptides have M r , of 129 687 (p130), 60 188 (p60) and 14 281 (p14). ORF 2 is thought to be expressed by suppression of the termination codon of ORF 1 to produce a M r , 189 975 product (p190). p130 contains sequences characteristic of proteins with methyl transferase and NTP-binding properties and p190 contains these and sequences characteristic of RNA-dependent RNA polymerases. The nucleotide sequence of IPCV RNA-1 is similar to that of peanut clump virus (PCV) and corresponding encoded polypeptides are 88% (p130), 95% p60 and 75% (p14) identical. The sequences of the translation products are also similar to those of soil-borne wheat mosaic virus and barley stripe mosaic virus. Oligonucleotide primers, designed on the basis of the sequences of RNA-1 of IPCV and PCV, were effective in reverse transcription-PCR amplification of these RNAs and that of IPCV isolates of the serologically distinct L and T serotypes.
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References
Brunt AA (1995) Genus Furovirus. In: Murphy FA, Fauquet CM, Bishop DHL, Ghabrial SA, Jarvis AW, Martelli GP, Mayo MA, Summers MD (eds) Virus Taxonomy. Classification and Nomenclature of Viruses: Sixth Report of the International Committee on Taxonomy of Viruses. Springer, Wien New York, pp 445–449 (Arch Virol [Suppl] 10)
Brunt AA, Richards KE (1989) Biology and molecular biology of furoviruses. Adv Virus Res 36: 1–32
Delfosse P, Reddy AS, Devi P, Murthy AK, Wesley SV, Naidu RA, Reddy DVR (1995) A disease of wheat caused by Indian peanut clump virus (IPCV). Plant Dis 79: 1074
Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395
Frohmann MA, Dush MK, Martin GR (1988). Rapid production of full-length cDNA from rare transripts: amplification using a single gene-specific oligonucleotide primer. Proc Natl Acad Sci USA 85: 8 998–9 002
Gubler U, Hoffman BJ (1983) A simple and very efficient method for generating cDNA libraries. J Mol Biol 227: 1–8
Herzog E, Guilley H, Manohar SK, Dollet M, Richards K, Fritsch C, Jonard G (1994) Complete nucleotide sequence of peanut clump virus RNA 1 and relationships with other fungus-transmitted rod-shaped viruses. J Gen Virol 75: 3 147–3 155
Macfarlane SA, Taylor SC, King DI, Hughes G, Davies JW (1989) Pea early browning virus RNA 1 encodes four polypeptides including a putative zinc-finger protein. Nucleic Acids Res 17: 2 245–2 260
Manohar SK, Guilley H, Dollet M, Richards K, Jonard G (1993) Nucleotide sequence and genetic organization of peanut clump virus RNA 2 partial characterization of deleted forms. Virology 195: 33–41
Manohar SK, Dollet M, Dubern J, Gargani D (1995) Studies on variability of peanut clump virus: symptomatology and serology. J Phytopathol 143: 233–238
Mayo MA, Reddy DVR (1985) Translation products of RNA from Indian peanut clump virus. J Gen Virol 66: 1 347–1 351
Mayo MA, Robinson DJ, Jolly CA, Hyman L (1989) Nucleotide sequence of potato leafroll luteovirus RNA. J Gen Virol 70: 1 037–1 051
Mayo MA, Miller JS, Wesley SV, Naidu RA, Reddy DVR (1993) Current research on Indian peanut clump virus at SCRI. In: Reddy DVR, McDonald D, Moss JP (eds) Working together on groundnut virus disease, ICRISAT, Patancheru, p 46
Nolt BL, Rajeshwari R, Reddy DVR, Bharatan N, Manohar SK (1988) Indian peanut clump virus isolates: host range, symptomatology, serological relationships, and some physical properties. Phytopathology 78: 310–313
Reddy DVR, Rajeshwari R, Iizuka N, Lesemann DE, Nolt BL, Goto T (1983) The occurrence of Indian peanut clump, a soil-borne virus disease of groundnuts (Arachis hypogaea) in India. Ann Appl Biol 102: 305–310
Reddy DVR, Robinson DJ, Roberts IM, Harrison BD (1985) Genome properties and relationships of Indian peanut clump virus. J Gen Virol 66: 2 011–2 016
Reddy DVR, Nolt BL, Hobbs HA, Reddy AS, Rajeshwari R, Rao AS, Reddy DDR, McDonald D (1988) Clump disease in India: isolates, host range, transmission and management. In: Cooper JI, Asher MJC (eds) Viruses with fungal vectors. Association of Applied Biologists, Wellesbourne, pp 239–246
Sanger F, Nicklen S, Coulson AR (1977) DNA sequencing with chain-terminating inhibitors. Proc Natl Acad Sci USA 74: 5 463–5 467
Shirako Y, Wilson TMA (1993) Complete nucleotide sequence and organization of the bi-partite RNA genome of soil-borne wheat mosaic virus. Virology 195: 16–32
Thouvenal JC, Fauquet C (1981) Further properties of peanut clump virus and studies on its natural transmission. Ann Appl Biol 97: 99–107
Wesley SV, Mayo MA, Jolly CA, Naidu RA, Reddy DVR, Jana MK, Parnaik VK (1994) The coat protein of Indian peanut clump virus: relationships with other furoviruses and with barley stripe mosaic virus. Arch Virol 134: 271–278
Wesley SV, Miller JS, Devi PS, Delfosse P, Naidu RA, Mayo MA, Reddy DVR, Jana MK (1996) Sensitive broad spectrum detection of Indian peanut clump virus by non-radioactive methods. Phytopathology (in press)
Zerfass K, Beier H (1992) The leaky UGA termination codon of tobacco rattle virus RNA is suppressed by tobacco chloroplast and cytoplasmic tRNAsTrp with CmCA anticodon. EMBO J 11: 4 167–4 173
Zhand H, Scholl R, Browse J, Somerville C (1988) Double stranded DNA sequencing as a choice for DNA sequencing. Nucleic Acids Res 16: 1 220
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The nucleotide sequence data reported in this paper has been deposited at EMBL under accession number X99149.
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Miller, J.S., Wesley, S.V., Naidu, R.A. et al. The nucleotide sequence of RNA-1 of Indian peanut clump furovius. Archives of Virology 141, 2301–2312 (1996). https://doi.org/10.1007/BF01718632
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DOI: https://doi.org/10.1007/BF01718632