Skip to main content
SpringerLink
Log in

Comparative amino acid sequence analysis of the major outer capsid protein (VP7) of porcine rotaviruses with G3 and G5 serotype specificities isolated in Venezuela and Argentina

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

Seven porcine group A rotavirus strains isolated in Venezuela were shown to be antigenically related to serotype G3 (five strains) or to serotype G5 (two strains), whereas two strains isolated in Argentina were classified as serotype G5. The serological classification of eight of these strains was confirmed by sequence analysis of the gene encoding the VP7 glycoprotein. A high degree of homology was observed among strains belonging to the same G serotype, although some variations in the serotype-specific regions were detected among different strains. Comparison with the published VP7 amino acid sequences of serotype G3 indicated that most porcine rotavirus strains are more closely related to each other and to human rotavirus strains than to rotavirus strains isolated from other species. Amino acid sequence comparison among serotype G5 porcine strains revealed that Venezuelan porcine isolates were more closely related to the American strain OSU, while the Argentinian strains had a higher similarity to the Australian strain TRF-41. This report confirms the worldwide distribution of these G serotypes among the porcine population.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  1. Bellinzoni RC, Mattion NM, Burrone O, Gonzalez A, La Torre JL, Scodeller EA (1987) Isolation of group A swine rotaviruses displaying atypical electropherotypes. J Clin Microbiol 25: 952–954

    Google Scholar 

  2. Bellinzoni RC, Mattion NM, Matson DO, Blackhall JO, La Torre JL, Scodeller EA, Urasawa S, Taniguchi K, Estes MK (1990) Porcine rotaviruses antigenically related to human rotavirus serotypes 1 and 2. J Clin Microbiol 28: 633–636

    Google Scholar 

  3. Blackhall J, Bellinzoni RC, Mattion NM, Estes MK, La Torre JL, Magnusson G (1992) A bovine rotavirus serotype 1: characterization of the virus and nucleotide sequence determination of the structural glycoprotein VP7 gene. Virology 189: 833–837

    Google Scholar 

  4. Bohl EH, Theil KW, Saif LJ (1984) Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol 19: 105–111

    Google Scholar 

  5. Browning GF, Chalmers RM, Fitzgerald TA, Snodgrass DR (1991) Serological and genomic characterization of L338, a novel equine group A rotavirus G serotype. J Gen Virol 72: 1059–1064

    Google Scholar 

  6. Browning GF, Fitzgerald TA, Chalmers RM, Snodgrass DR (1991) A novel group A rotavirus G serotype: serological and genomic characterization of equine isolate F123. J Clin Microbiol 29: 2043–2046

    Google Scholar 

  7. Browning GF, Chalmers RM, Fitzgerald TA, Corley KTT, Campbell I, Snodgrass DR (1992) Rotavirus serotype G3 predominates in horses. J Clin Microbiol 30: 59–62

    Google Scholar 

  8. Caust J, Dyall-Smith ML, Lazdins I, Holmes IH (1987) Glycosylation, an important modifier of rotavirus antigenicity. Arch Virol 96: 123–134

    Google Scholar 

  9. Ciarlet M, Liprandi F (1994) Serological and genomic characterization of two porcine rotaviruses with serotype G1 specificity. J Clin Microbiol 32: 269–272

    Google Scholar 

  10. Ciarlet M, Hidalgo M, Gorziglia M, Liprandi F (1994) Characterization of neutralization epitopes on VP7 of serotype G11 porcine rotaviruses. J Gen Virol 75: 1867–1873

    Google Scholar 

  11. Dyall-Smith ML, Lazdins I, Tregear GW, Holmes IH (1986) Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci USA 83: 3465–3468

    Google Scholar 

  12. Estes MK, Cohen J (1989) Rotavirus gene structure and function. Microbiol Rev 53: 410–449

    Google Scholar 

  13. Glass RI, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian AZ, Chanock RM, Flores J (1985) Nucleotide sequence of the structural glycoprotein VP7 gene of the Nebraska calf diarrhoea virus rotavirus: comparison with homologous genes from four strains of human and animal rotaviruses. Virology 141: 292–298

    Google Scholar 

  14. Gorziglia M, Aguirre Y, Hoshino Y, Esparza J, Blumentals I, Askaa J, Thompson M, Glass RI, Kapikian AZ, Chanock RM (1986) VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. J Gen Virol 67: 2445–2454

    Google Scholar 

  15. Gorziglia M, Nishikawa K, Green KY, Taniguchi K (1988) Gene sequence of the VP7 serotype specific glycoprotein of Gottfried porcine rotavirus. Nucleic Acids Res 16: 775

    Google Scholar 

  16. Gorziglia M, Nishikawa K, Hoshino Y, Taniguchi K (1990) Similarity of the outer capsid protein VP4 of the Gottfried strain of porcine rotavirus to that of asymptomatic human rotavirus strains. J Virol 64: 414–418

    Google Scholar 

  17. Gouvea V, De Castro L, Do Carmo Timenetsky M, Greenberg HB, Santos N (1994) Rotavirus serotype G 5 associated with diarrhoea in Brazilian children. J Clin Microbiol 32: 1408–1409

    Google Scholar 

  18. Green KY, Midthun K, Gorziglia M, Hoshino Y, Kapikian AZ, Chanock RM, Flores J (1987) Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161: 153–159

    Google Scholar 

  19. Greenberg HB, McAuliffe V, Valdesuso J, Wyatt R, Flores J, Kalica A, Hoshino Y, Singh N (1983) Serological analysis of the subgroup protein of rotavirus using monoclonal antibodies. Infect Immun 39: 91–99

    Google Scholar 

  20. Higgins DG, Sharp PM (1989) Fast and sensitive multiple sequence alignments on a microcomputer. CABIOS 5: 151–153

    Google Scholar 

  21. Hoshino Y, Wyatt R, Greenberg HB, Flores J, Kapikian AZ (1984) Serotypic similarity and diversity of rotavirus of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis 149: 694–702

    Google Scholar 

  22. Hoshino Y, Sereno MM, Midthun K, Flores J, Kapikian AZ, Chanock RM (1985) Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci USA 82: 8701–8704

    Google Scholar 

  23. Hoshino Y, Nishikawa K, Benfield DA, Gorziglia M (1994) Mapping of antigenic sites involved in serotype-cross-reaction neutralization on group A rotavirus outercapsid glycoprotein VP7. Virology 199: 233–237

    Google Scholar 

  24. Huang J, Nagesha HS, Dyall-Smith ML, Holmes IH (1989) Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Arch Virol 109: 173–183

    Google Scholar 

  25. Huang J-A, Nagesha HS, Holmes IH (1993) Comparative sequence analysis of VP4s from five Australian porcine rotaviruses: implication of an apparent new P type. Virology 196: 319–327

    Google Scholar 

  26. Hussein HA, Parwani AV, Rosen BI, Lucchelli A, Saif LJ (1993) Detection of rotavirus serotypes G1, G2, G3, and G11 in feces of diarrheic calves by using polymerase chain reaction-derived cDNA probes. J Clin Microbiol 31: 2491–2496

    Google Scholar 

  27. Kang S-Y, Saif LJ, Miller K (1989) Reactivity of VP4-specific monoclonal antibodies to a serotype 4 porcine rotavirus with distinct serotype of human (symptomatic and asymptomatic) and animal rotaviruses. J Clin Microbiol 27: 2744–2750

    Google Scholar 

  28. Kool DA, Holmes IH (1993) The avian rotavirus Ty-1 VP7 nucleotide and deduced amino acid sequences differ significantly from those of Ch-2 rotavirus. Arch Virol 129: 227–234

    Google Scholar 

  29. Li B, Gorziglia M (1993) VP4 serotype of the Gottfried strain of procine rotavirus. J Clin Microbiol 31: 3075–3077

    Google Scholar 

  30. Liprandi F, López G, Rodriguez I, Hidalgo M, Ludert JE, Mattion NM (1990) Monoclonal antibodies to the VP6 of porcine subgroup I rotaviruses reactive with subgroup I and non-subgroup II strains. J Gen Virol 71: 1395–1398

    Google Scholar 

  31. Liprandi F, Rodriguez I, Piña CI, Larralde G, Gorziglia M (1991) VP4 monotype specificities among porcine rotaviruses strains of the same VP4 serotype. J Virol 65: 1658–1661

    Google Scholar 

  32. Mattion NM, González SA, Burrone O, Bellinzoni RC, La Torre JL, Scodeller EA (1988) Rearrangement of genomic segment 11 in two swine rotavirus strains. J Gen Virol 69: 695–698

    Google Scholar 

  33. Mattion NM, Bellinzoni RC, Blackhall JO, La Torre JL, Scodeller EA (1989) Antigenic characterization of swine rotaviruses in Argentina. J Clin Microbiol 27: 795–798

    Google Scholar 

  34. Nagesha HS, Holmes IH (1988) New porcine rotavirus serotype antigenically related to human rotavirus serotype 3. J Virol 175: 171–174

    Google Scholar 

  35. Nagesha HS, Huang J, Hum CP, Holmes IH (1990) A porcine rotavirus strain with dual VP7 serotype specificity. Virology 175: 319–322

    Google Scholar 

  36. Nagesha HS, Holmes IH (1991) Direct serotyping of porcine rotaviruses using VP7-specific monoclonal antibodies by an enzyme immunoassay. J Med Virol 35: 206–211

    Google Scholar 

  37. Nagesha HS, Huang J, Holmes IH (1992) A variant serotype G3 rotavirus isolated from an unusually severe outbreak of diarrhoea in piglets. J Med Virol 38: 79–85

    Google Scholar 

  38. Nishikawa K, Fukuhara N, Liprandi F, Green K, Kapikian AZ, Chanock RM, Gorziglia M (1989) VP4 protein of porcine rotavirus strain OSU expressed by a baculovirus recombinant induces neutralizing antibodies. Virology 173: 631–637

    Google Scholar 

  39. Nishikawa K, Hoshino Y, Taniguchi K, Green KY, Greenberg HB, Kapikian AZ, Chanock RM, Gorziglia M (1989) Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology 171: 503–515

    Google Scholar 

  40. Offit PA, Blavat G (1986) Identification of the two rotavirus genes determining neutralization specificities. J Virol 57: 376–378

    Google Scholar 

  41. Paul PS, Lyoo YS, Andrews JJ, Hill HT (1988) Isolation of two new serotypes of porcine rotavirus from pigs with diarrhoea. Arch Virol 100: 139–143

    Google Scholar 

  42. Rosen BI, Parwani AV, López S, Flores J, Saif LJ (1994) Serotypic differentiation of rotaviruses in field samples from diarrheic pigs by using nucleic acid probes specific for porcine VP4 and human and porcine VP7 genes. J Clin Microbiol 32: 311–317

    Google Scholar 

  43. Ruiz AM, López IV, López S, Espejo RT, Arias CF (1988) Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. J Virol 62: 4331–4336

    Google Scholar 

  44. Saif LJ, Rosen BI, Parwani AV (1994) Animal rotaviruses. In: Kapikian AZ (ed) Viral infections of the gastrointestinal tract. Marcel Dekker, New York Basel Hong Kong, pp 279–367

    Google Scholar 

  45. Sereno MM, Gorziglia MI (1994) The outer capsid VP4 of murine rotavirus strain Eb represents a tentative new P type. Virology 199: 500–504

    Google Scholar 

  46. Taniguchi K, Urasawa T, Kobayashi N, Gorziglia M, Urasawa S (1990) Nucleotide sequence of VP4 and VP7 genes of human rotaviruses with subgroup 1 specificity and long RNA pattern: implication for new G serotype specificity. J Virol 64: 5640–5644

    Google Scholar 

  47. Taniguchi K, Urasawa T, Urasawa S (1994) Species specificity and interspecies relatedness in VP4 genotype demonstrated by VP4 sequence analysis of equine, feline, and canine rotavirus strains. Virology 200: 390–400

    Google Scholar 

  48. Webster RG, Laver WG, Air GM, Schild GC (1982) Molecular mechanisms of variation of influenza virus. Nature 296: 115–121

    Google Scholar 

Download references

Author information

Author notes

  1. M. Ciarlet

    Present address: Department of Molecular Virology, Baylor College of Medicine, Houston, TX

  2. J. E. Ludert

    Present address: Division of Gastroenterology, Stanford University School of Medicine, Palo Alto, CA, USA

Authors and Affiliations

  1. Lab. Biología de Virus, Centro de Microbiología y Biología Celular, Instituto Venezolano de Investigaciones Científicas, Caracas, Venezuela

    M. Ciarlet, J. E. Ludert & F. Liprandi

Authors
  1. M. Ciarlet
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. J. E. Ludert
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. F. Liprandi
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Ciarlet, M., Ludert, J.E. & Liprandi, F. Comparative amino acid sequence analysis of the major outer capsid protein (VP7) of porcine rotaviruses with G3 and G5 serotype specificities isolated in Venezuela and Argentina. Archives of Virology 140, 437–451 (1995). https://doi.org/10.1007/BF01718422

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01718422

Keywords

Search

Navigation