Skip to main content
SpringerLink
Log in

Epidemiological markers of coagulase-negative staphylococci

  • Review Articles
  • Published:
Intensive Care Medicine Aims and scope Submit manuscript

Abstract

Several different epidemiological typing methods have been used in studies of coagulase-negative staphylococci; these include biotyping, antibiotic susceptibility pattern analysis, serological typing, phage typing, slime production detection, protein profile analysis, immunoblot fingerprinting and DNA typing. The most frequently used tests are antibiotic susceptibility and extrachromosomal DNA banding patterns. Comparison of chromosomal DNA restriction patterns is facilitated when the fragments are separated by pulsed-field electrophoresis. Typing variations occur quite frequently even in isolates of demonstrated clinical significance. Caution should therefore be exercised in denying the clinical relevance of coagulase-negative staphylococci, even when successive isolates show distinct typing differences.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Pfaller MA, Herwaldt LA (1988) Laboratory, clinical, and epidemiological aspects of coagulase-negative staphylococci. Clin Microbiol Rev 1:281–289

    PubMed  Google Scholar 

  2. Archer GL (1985) Coagulase negative staphylococci in blood cultures: the clinician's dilemna. Infect Control 6:477–478

    PubMed  Google Scholar 

  3. Kirchhoff LV, Sheagren JN (1985) Epidemiology and clinical significance of blood cultures positive for coagulase negativeStaphylococcus. Infect Control 6:479–486

    PubMed  Google Scholar 

  4. Etienne J, Brun Y, El Solh N, Delorme V, Mouren C, Bes M, Fleurette J (1988) Characterization of clinically significant isolates ofStaphylococcus epidermidis isolated from patients with endocarditis. J Clin Microbiol 26:613–617

    PubMed  Google Scholar 

  5. Etienne J, Charpin B, Grando J, Brun Y, Bes M, Fleurette J (1991) Characterization of clinically significant isolates ofStaphylococcus epidermidis from patients with cerebrospinal fluid shunt infections. Epidemiol Infect 106:467–475

    PubMed  Google Scholar 

  6. Parisi JT, Lampson BC, Hoover DL, Khan JA (1986) Comparison of epidemiologic markers forStaphylococcus epidermidis. J Clin Microbiol 24:56–60

    PubMed  Google Scholar 

  7. Christensen GD, Parisi JT, Bisno AL, Simpson WA, Beachey EH (1983) Characterization of clinically significant strains of coagulase negative staphylococci. J Clin Microbiol 18:258–269

    PubMed  Google Scholar 

  8. Fleurette J, Bes M, Brun Y, Freney J, Forey F, Coulet M, Reverdy ME, Etienne J (1989) Clinical isolates ofStaphylococcus lugdunénsis andS. schleiferi: bacteriological characteristics and susceptibility to antimicrobial agents. Res Microbiol 140:107–118

    PubMed  Google Scholar 

  9. Low DE, Schmidt BK, Kirplani HM, Moodie R, Kreiswirth B, Matlow A, Ford-Jones EL (1992) An endemic strain ofStaphylococcus haemolyticus colonizing and causing bacteremia in neonatal intensive care unit patients. Pediatrics 89:696–700

    PubMed  Google Scholar 

  10. Mickelsen PA, Plorde JJ, Gordon KP, Hargiss C, McClure J, Schoenknecht FD, Condie F, Tenover FC, Tompkins LS (1985) Instability of antibiotic resistance in a strain ofStaphylococcus epidermidis isolated from an outbreak of coagulase-negative staphylococci. J Infect Dis 152:50–58

    PubMed  Google Scholar 

  11. Etienne J, Renaud F, Bes M, Brun Y, Greenland TB, Freney J, Fleurette J (1990) Instability of characteristics amongst coagulasenegative staphylococci causing endocarditis. J Med Microbiol 32:115–122

    PubMed  Google Scholar 

  12. Christensen GD, Baddour LM, Madison BM, Parisi JT, Abraham SN, Hasty DL, Lowrance JH, Josephs JJ, Simpson WA (1990) Colonial morphology of staphylococci on memphis agar: phase variation of slime production, resistance to β-lactam antibiotics, and virulence. J Infect Dis 161:1153–1169

    PubMed  Google Scholar 

  13. Schumacher-Perdreau F, Jansen B, Peters G, Pulverer G (1988) Typing of coagulase-negative staphylococci isolated from foreign body infections. Eur J Clin Microbiol Infect Dis 7:270–273

    PubMed  Google Scholar 

  14. Burnie JP, Lee W (1988) A comparison of DNA and immunoblot fingerprinting of SII biotype of coagulase-negative staphylococci. Epidemiol Infect 101:203–212

    PubMed  Google Scholar 

  15. Renaud F, Etienne J, Bertrand A, Brun Y, Greenland TB, Freney J, Fleurette J (1991) Molecular epidemiology ofStaphylococcus haemolyticus strains isolated in an Albanian hospital. J Clin Microbiol 29:1493–1497

    PubMed  Google Scholar 

  16. Bialkowska-Hobrzanska H, Jaskot D, Hammerberg L (1990) Evaluation of restriction endonuclease fingerprinting of chromosomal DNA and plasmid profile analysis for characterization of multiresistant coagulase-negative staphylococci in bacteremic neonates. J Clin Microbiol 28:269–275

    PubMed  Google Scholar 

  17. Etienne J, Poitevin-Later F, Renaud F, Fleurette J (1990) Plasmid profiles and genomic DNA restriction endonuclease patterns of 30 independentStaphylococcus lugdunensis strains. FEMS Microbiol Lett 67:93–98

    Google Scholar 

  18. Poitevin-Later F, Vandenesch F, Dyke K, Fleurette J, Etienne J (1992) Cadmium-resistance plasmid inStaphylococcus lugdunensis. FEMS Microbiol Lett 99:59–62

    Google Scholar 

  19. Etienne J, Grattard F, Poitevin-Later F, Vandenesch F, Fleurette J (1990) Plasmid profiles of 60 independent strains ofStaphylococccus lugdunensis andStaphylococcus schleiferi. Abstr Annu Meet Am Soc Microbiol C350 p 402

    Google Scholar 

  20. Renaud J, Freney J, Etienne J, Bes M, Brun Y, Barsotti O, André S, Fleurette J (1988) Restriction endonuclease analysis ofStaphylococcus epidermidis DNA may be a useful epidemiological marker. J Clin Microbiol 26:1729–1734

    PubMed  Google Scholar 

  21. Lina B, Vandenesch F, Etienne J, Kreiswirth B, Fleurette J (1992) Comparison of coagulase-negative staphylococci by pulsed-field gel electrophoresis. FEMS Microbiol Lett 92:133–138

    Google Scholar 

  22. Prévost G, Pottecher B, Dahlet M, Bientz M, Mantz JM, Piémont Y (1991) Pulsed field electrophoresis as a new epidemiological tool for monitoring methicillin-resistantStaphylococcus aureus in an intensive care unit. J Hosp Infect 17:255–269

    PubMed  Google Scholar 

  23. Lina B, Bès M, Vandenesch F, Fleurette J, Etienne J (1992) Detection of genomic instability of coagulase-negative staphylococci strains by pulsed-field gel electrophoresis. Abstr VIIth International Symposium on Staphylococci and Staphylococcal Infections no 130

  24. De Buyser ML, Morvan A, Grimont F, El Solh N (1989) Characterization ofStaphylococcus species by ribosomal RNA gene restriction patterns. J Gen Microbiol 135:989–999

    PubMed  Google Scholar 

  25. Thomson-Carter F, Carter PE, Pennington TH (1989) Differentiation of staphylococcal species and strains by ribosomal RNA gene restrictions patterns. J Gen Microbiol 135:2093–2097

    PubMed  Google Scholar 

  26. Bialkowska-Hobrzanska H, Harry V, Jaskot D, Hammerberg O (1990) Typing of coagulase-negative staphylococci by southern hybridization of chromosomal DNA fingerprints using ribosomal RNA probe. Eur J Clin Microbiol Infect Dis 9:588–594

    PubMed  Google Scholar 

  27. De Buyser ML, Morvan A, Aubert S, Dilasser F, El Solh N (1992) Evaluation of ribosomal RNA gene probe for the identification of species and subspecies within the genusStaphylococcus. J Gen Microbiol 138:889–899

    PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. BP Lyon Montchat, Hôpital Louis Pradel, F-69394, Lyon, France

    F. Vandenesch, B. Lina, Ch. Lebeau, Th. B. Greenland &  J. Etienne

Authors
  1. F. Vandenesch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. B. Lina
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Ch. Lebeau
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Th. B. Greenland
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. J. Etienne
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Vandenesch, F., Lina, B., Lebeau, C. et al. Epidemiological markers of coagulase-negative staphylococci. Intensive Care Med 19, 311–315 (1993). https://doi.org/10.1007/BF01694703

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01694703

Key words

Search

Navigation