Abstract
Wax deposits on leaf surfaces ofin vitro-grown plantlets,in vitro plantlets treated with polyethylene glycol and greenhouse-grown seedlings from five cultivars of date palm (Phoenix dactylifera L.) were extracted and quantified. Significant variations among treatments and cultivars were obtained. Greenhouse-grown plants had the greatest wax deposits followed by the acclimatized plantlets.In vitro plantlets had an average of 15% of the wax of greenhouse plants. Cultivar and plant age differences had a significant effect on the quantity of wax deposits. Greenhouse seedlings of ’Majhool’, ’Deglet Noor’ and ’Khadraoui’ (cultivars grown under irrigation) had less wax accumulation than ’Zahidi’ and ’Sayer’, dryland cultivars.
The increase in wax deposition as a result of polyethylene glycol treatment, explains in part, the decreased water loss observed in acclimatized plantlets when transferredex vitro.
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Abbreviations
- EW:
-
epicuticular wax
- NAA:
-
naphthalene acetic acid
- PEG:
-
polyethylene glycol
References
Brainerd KE, Fuchigami LH (1982) 107 Annual Convention of the Amer. Assoc. of Nurserymen. Honolulu, Hawaii, pp. 23–25
Dahlgren BE (1933) Tropical Woods 35:3–5
Dami I, Hughes HG (1991) HortScience 26:725 (Abstr.)
Debergh PC (1991) Acta Horticulturae 289:291–300
Debergh PC, Zimmerman RH (1990) Micropropagation technology and application. Kluwer Academic Publishers, Dordrecht
Denna DW (1970) Aust. J. Biol. Sci. 23:27–31
Dhawan V, Bhojwani SS (1987) Plant Science 53:65–72
Fuchigami LH, Cheng TY, Soeldner A (1981) J. Amer.Soc. Hort. Sci. 106:519–522
Galeano R, Rumbaugh DM, Johnson DA (1986) Crop Science 26:703–405
Grout BW, Aston MJ (1977) Hortic. Res. 17:1–7
Jordan WR, Monk RL, Shouse PJ (1983) Crop Sci. 23:552–558
Jordan WR, Shouse PJ, Monk RL (1984) Crop. Sci. 24:1168–1173
Lewandowski VT (1991) HortScience 26:586–589
McClelland MT, Smith MAL, Carothers ZB (1990) Plant Cell, Tissue and Organ Culture 23:115–123
McNair JB (1951) Amer. J. Bot. 18:518–525
Murashige T (1978) In: Frontiers of Plant Tissue Culture. T.A. Thorpe, ed. Univ. Calgary Press, Alberta, Canada, pp. 15–26; 518–524
Murashige T, Skoog F (1962) Physiol. Plant 15:473–497
Okada M, Ozawa K, Hamasaki T (1992) Acta Horticulturae 319:159–162
Preece JE, Sutter EG (1991) In: Debergh PC, Zimmerman RH (eds.). Micropropagation technology and application: Kluwer Academic Publishers, Dordrecht, pp. 71–93
Premachandra GS, Saneoka H, Kanaya M, Ogata S (1991) J. Exp. Bot. 42(235):167–171
Safadi F (1992) Ph.D. Dissertation. Colorado State University, Fort Collins, CO
Short KC, Warburton J, Roberts AV (1987) Acta Horticulturae 212:329–334
Sutter EG (1988) J. Amer. Soc. HortSci. 113:234–238
Sutter EG, Langhans RW (1980) HortScience 15(3):97–98
Sutter EG, Langhans RW (1982) Can. J. Bot. 60:2896–2902
Sutter EG, Novello V, Shackel K (1988) Acta Horticulturae 230:113–119
Wardle K, Dobbs EB, Short KC (1983) J. Amer. Soc. Hort. Sci. 108(3):386–389
Zaid A (1990) Ph.D. Dissertation. Colorado State University, Fort Collins, CO
Ziv M (1986) In: L.A. Withers and P.G. Alderson (eds.) Plant tissue culture and its agricultural applications. Butterworths, London, pp. 187–196
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Communicated by A. Komamine
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Zaid, A., Hughes, H. In vitro acclimatization of date palm (Phoenix dactylifera L.) plantlets: A quantitative comparison of epicuticular leaf wax as a function of polyethylene glycol treatment. Plant Cell Reports 15, 111–114 (1995). https://doi.org/10.1007/BF01690265
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DOI: https://doi.org/10.1007/BF01690265