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Differentiation of histochemically visualized mercury and silver

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Summary

Accumulations of silver and mercury can be visualized in tissue sections by a technique called autometallography or physical development. In order to make a histological differentiation between mercury and silver in tissue exposed to both metals, it is necessary to remove one of the metals while leaving the other untouched. The present paper describes a technique by which silver accumulations in histological sections can be removed by potassium cyanide, yet leaving mercury accumulations intact to be developed autometallographically.

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References

  • AASETH, J., OLSEN, A., HALSE, J. & HOVIG, T. (1981) Argyria-tissue deposition of silver as selenide.Scand. J. clin. Lab. Invest. 41 247–51.

    Google Scholar 

  • DANSCHER, G. (1981a) Histochemical demonstration of heavy metals. A revised version of the sulphide silver method suitable for both light and electron microscopy.Histochemistry 71 1–16.

    Google Scholar 

  • DANSCHER, G. (1981b) Light and electron microscopic localization of silver in biological tissue.Histochemistry 71 177–86.

    Google Scholar 

  • DANSCHER, G. (1982) Exogenous selenium in the brain. A histochemical technique for light and electron microscopical localization of catalytic selenium bonds.Histochemistry 76 281–93.

    Google Scholar 

  • DANSCHER, G. (1984) Autometallography.Histochemistry 81 331–5.

    Google Scholar 

  • DANSCHER, G., HOWELL, G., PEREZ-CLAUSELL, J. & HERTEL, N. (1985) The dithizone, Timm's sulphide silver and the selenium methods demonstrate a chelatable pool of zinc in CNS.Histochemistry 83 419–22.

    Google Scholar 

  • DANSCHER, G. & MØLLER-MADSEN, B. (1985) Silver amplification of mercury sulfide and selenide.J. Histochem. Cytochem. 33 219–28.

    Google Scholar 

  • DANSCHER, G. & ZIMMER, J. (1978) An improved Timm sulphide silver method for light and electron microscopic localization of heavy metals in biological tissues.Histochemistry 55 27–40.

    Google Scholar 

  • DEMPSEY, E. W. (1973) Neural and vascular ultrastructure of the area postrema of the rat.J. comp. Neurol. 150 177–200.

    Google Scholar 

  • JAMES, T. H. (1939) The reduction of silver ions by hydroquinone.J. Am. Chem. Soc. 61 648–52.

    Google Scholar 

  • JAMES, T. H. (1977)The Theory of the Photographic Process. New York: Macmillan.

    Google Scholar 

  • LIESEGANG, R. (1928) Histologische Versilberung.Z. wiss. Mikrosk. 45 273–9.

    Google Scholar 

  • RUNGBY, J., DANSCHER, G., MØLLER-MADSEN, B., RESKENIELSEN, E. & HANSEN, J. C. (1983) Localization of silver and mercury in human brain tissue.Neurosci. Letts., suppl. 14, 318.

    Google Scholar 

  • SHIRABE, T. (1979) Identification of mercury in the brain of Minamata disease victims by electron microscopic X-ray microanalysis.Neurotoxicology 1 349.

    Google Scholar 

  • TIMM, F. (1958) Zur Histochemie der Schwermetalle. Das Sulfid-Silber-Verfahren.Dtsch. Z. Gesamte Gerichtl. Med. 46 706–11.

    Google Scholar 

  • TIMM, F. (1962) Der histochemische Nachweis der Sublimatvergiftung.Beitr. Gerichtl. Med. 21 195–7.

    Google Scholar 

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Authors and Affiliations

  1. Institute of Anatomy B, University of Aarhus, DK-8000, Aarhus C, Denmark

    G. Danscher & J. Rungby

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  1. G. Danscher
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  2. J. Rungby
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Danscher, G., Rungby, J. Differentiation of histochemically visualized mercury and silver. Histochem J 18, 109–114 (1986). https://doi.org/10.1007/BF01675364

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  • DOI: https://doi.org/10.1007/BF01675364

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