Summary
Acetic acid extracts of bovine pineals and cerebral cortex were separated on Sephadex G-25 columns. Subsequently two low molecular weight fractions, F2 and F3, were ultrafiltered through the membranes UM2 and UM05. The UM05 residues were gel filtered on Sephadex G-15 columns or chromatographed on Dowex W50-X4 columns. Fractions from these columns were tested and those which showed COH-inhibiting activity were separated by preparative paper chromatography in different solvents. The absorption spectra of those fractions were recorded and tested for COH-inhibition. By these methods, a COH-inhibitor was localized in three different solvents. Some active paper chromatography fractions were studied in high pressure, reverse phase, liquid chromatography. This latter method showed that the active fractions obtained by paper chromatography contain several orthophthalaldehyde (OPT) positive compounds.
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Aures, D., Fleming, R., Håkanson, R. Microanalysis of tissue amines and enzymes involved in their metabolism. Separation and detection of biogenic amines by thinlayer chromatography. J. Chrom.33, 480–493 (1968).
Bensinger, R., Vaughan, M., Klein, D. C. Isolation of a non-melatonin lipophilic antigonadotropic factor from the bovine pineal gland. Fed. Proc.32, 25 (1973).
Benson, B., Orts, R. J. Regulation of ovarian growth by the pineal gland. In: Regulation of Organ and Tissue Growth (Goss, R. J., ed.), pp. 315 to 332. New York-London: Academic Press. 1972.
Benson, B., Matthews, M. J., Hruby, V. J. Characterization and effects of a bovine antigonadotropic peptide. Am. Zool.16, 17–24 (1976 a).
Benson, B., Matthews, M. J., Orts, R. J. Presence of an antigonadotropic substance in rat pineal incubation media. Life Sci.11, 669–677 (1972 a).
Benson, B., Matthews, M. J., Rodin, A. E. Studies on a non-melatonin pineal antigonadotropin. Acta Endocrinol.69, 257–266 (1972 b).
Benson, B., Matthews, M. J., Hadley, M. E., Powers, S., Hruby, V. J. Differential localization of antigonadotropic and vasotocic activities in bovine and rat pineal. Life Sci.19, 747–754 (1976 b).
Ebels, I., Benson, B. A survey of the evidence that unidentified pineal substances affect the reproductive system in mammals. Prog. Reprod. Biol.4, 48–87 (1978).
Ebels, I., Benson, B., Matthews, M. J. Localization of a sheep pineal antigonadotropin. Anal. Biochem.56, 546–565 (1973).
Meek, J. L. Application of inexpensive equipment for high pressure liquid chromatography to assays for taurine,γ-amino butyric acid and 5-hydroxy-tryptophan. Anal. Chem.48, 375–379 (1976).
Orts, R. J., Benson, B. Inhibitory effects on serum and pituitary LH by a melatonin free-extract of bovine pineal glands. Life Sci.12, 513–519 (1973).
Orts, R. J., Benson, B., Cook, B. F. Some antigonadotropic effects of melatonin-free bovine pineal extracts. Acta Endocr.76, 438–448 (1974).
Rosenblum, I. Y., Benson, B., Bria, C. F., McDonnell, D., Hruby, V. J.: Localization and chemical characterization of a partially purified bovine pineal antigonadotropin. (Submitted for publication, 1978.)
Rosenblum, I. Y., Benson, B., Hruby, V. J. Chemical differences between bovine pineal antigonadotropin and arginine vasotocin. Life Sci.18, 1367–1374 (1976).
Vaughan, M. K., Benson, B., Norris, J. T., Vaughan, G. M. Inhibition of compensatory ovarian hypertrophy in mice by melatonin, 5-hydroxy-tryptamine and pineal powder. J. Endocrinol.50, 171–175 (1971 a).
Vaughan, M. K., Reiter, R. J., Vaughan, G. M. Inhibition of compensatory, ovarian hypertrophy by Altschule's pineal extract, pineal indoles and vasopressin in mice and voles. Am. Zool.2, 645–650 (1971 b).
Vaughan, M. K., Reiter, R. J., Vaughan, G. M., Bigelow, L., Altschule, M. D. Inhibition of compensatory ovarian hypertrophy in the mouse and vole: a comparison of Altschule's pineal extract, pineal indoles, vasopressin and oxytocin. Gen. Comp. Endocr.18, 372–377 (1972).
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This work was supported by NIH Grant no. HD 08759.
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Ebels, I., Benson, B., Bria, C.F. et al. Location by paper chromatography of compensatory ovarian hypertrophy (COH) inhibiting activity in acetic acid extracts from bovine pineals. J. Neural Transmission 42, 275–292 (1978). https://doi.org/10.1007/BF01673552
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DOI: https://doi.org/10.1007/BF01673552