Summary
The carcinogenicity of long-term feeding of masheri extract to animals in a vitamin-A-sufficient (SLO+) and deficient (SLO−) state was studied in Sprague Dawley rats by feeding daily dose of 3 mg extract over a period of 21 mounths. The phase I activating enzymes, the glutathione (GSH)/glutathione S-transferase (GST) detoxification system, and the hepatic and circulating levels of vitamins A and C were also monitored at 12 and 21 months. It was observed that the phase I enzyme activities were significantly higher in SLO+ than in SLO− rats at both 12 months and 21 months. Moreover, the SLO− masheri-treated animals also showed a decreased in the GSH/GST detoxification system while the reverse was observed in SLO+ group. Masheri extract treatment significantly lowered the hepatic and circulating levels of vitamin A while a concurrent increase was observed in the vitamin C level. The extract was found to be tumorigenic in both the SLO+ and SLO− groups. Benign tumours were observed in the SLO+ group while a high incidence of malignant tumours of the lung were observed in the SLO− group upon treatment with masheri extract.
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Abbreviations
- SD:
-
Sprague Dawley
- SLO+ :
-
diet sufficient in vitamin A
- SLO:
-
diet deficient in vitamin A
- GSH:
-
glutathione
- GST:
-
glutathioneS-transferase
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Ammigan, N., Nair, U.J., Lalitha, V.S. et al. Carcinogenicity studies of masheri: pyrolysed tobacco product, in vitamin-A-deficient sprague dawley rats. J Cancer Res Clin Oncol 117, 50–54 (1991). https://doi.org/10.1007/BF01613196
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DOI: https://doi.org/10.1007/BF01613196