Summary
The expression of PA8-15 antigen and the blood-group-related antigens A, B, O, Lea, Leb, Lex, and Ley, as well as CA19-9, were examined in the normal pancreas and in specimens from benign and malignant pancreatic tissue by the avidin-biotin-immunoperoxidase technique. A correlation was found between the expression of PA8-15, Lea, and CA19-9 in some cases. However, in the cancer tissues in which neither Lea nor CA19-9 could be demonstrated, strong expression of PA8-15 was observed. The reactivity of monoclonal antibody (mAb) PA8-15 with pancreatic cancer tissue was not inhibited by the preincubation of the sections with the mAb anti-Lea (CO514) and mAb CA19-9 (CO19-9) indicating that the epitope recognized by PA8-15 is different from that detected by the other two antibodies. Moreover, unlike Lea and CA19-9, PA8-15 was also expressed in cancer cells of patients of the Lea- b- type. The results suggest that mAb PA8-15 recognizes a sialylated molecule related to Lea but different from CA19-9, and seems to be an additional useful marker for pancreatic cancer.
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Abbreviations
- mAb:
-
monoclonal antibody
- BGRA:
-
blood-group-related antigens
- CEA:
-
carcinoembryonic antigen
- ABC:
-
avidin-biotin-peroxidase complex
References
Blaszcyk M, Hanson GC, Karlsson KA, Larson G, Stromberg N, Thurin J, Herlyn M, Steplewski Z, Koprowski H (1984) Lewis blood-group antigens defined by monoclonal anti-colon carcinoma antibodies. Arch Biochem Biophys 223:161–168
Borowitz MJ, Tuck FL, Sideler WF, Fernsten PD, Metzger RS (1984) Monoclonal antibodies against human pancreatic adenocarcinoma: distribution of DU-PAN-2 antigen on glandular epithelia and adenocarcinomas. J Natl Cancer Inst 72:999–1005
Brockhaus M, Magnani ML, Blaszczyk M, Steplewski Z, Koprowski H, Karlsson KA (1981) Monoclonal antibodies against human Le blood-group antigen. Biol Chem 256:13223–13225
Ernst C, Atkinson B, Wysocka M, Blaszczyk M, Herlyn M, Sears H, Steplewski Z, Koprowski H (1984) Monoclonal antibody localization of Lewis antigens in fixed tissue. Lab Invest 50:394–400
Gold DV, Hollingsworth P, Kremer T, Nelson D (1983) Identification of human pancreatic duct tissue specific antigen. Cancer Res 43:235–238
Graham HA, Hirsch HF, Davis DM (1977) Genetic and immunochemical relationships between soluble and cell-bound antigens of the Lewis system. In: Cunningham M, Plunkell L (eds) Human blood groups. Karger, Basel, pp 150–163
Herlyn M, Sears HF, Steplewski Z, Koprowski H (1982) Monoclonal antibody detection of a circulating tumor-associated antigen. I. Presence of antigen in sera of patients with colorectal, gastric and pancreatic carcinoma. J Clin Immunol 2:135–140
Hsu SM, Raine L, Fanger H (1988) Use of avidin-biotin-peroxidase complex (ABC) in immuno-peroxidase technique: a comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Itai S, Arii S, Tobe R, Kitahara A, Kim YC, Yamabe H, Ohtsuki H, Kirihara Y, Shigeta K, Kannagi R (1988) Significance of 2–3 and 2–6 sialylation of Lewis A antigen in pancreas cancer. Cancer 61:775–787
Itzkowitz SH, Yuan M, Ferell LD, Ratcliffe RM, Chung Ys, Satake K, Umeyama K, Jones RT, Kim YS (1987) Cancer-associated alterations of blood group antigen expression in the human pancreas. J Natl Cancer Inst 79:425–434
Itzkowitz SH, Yuan M, Fukushi Y, Lee H, Shi Z, Zurawski V, Hakomori S, Kim YS (1988) Immunohistochemical comparison of Lea, monosialosyl Lea (CA19-9), and disialosyl Lea antigens in human colorectal and pancreatic tissues. Cancer Res 48:3834–3842
Iwamura T, Katsuki T, Ide K (1987) Establishment and characterization of a human pancreatic cancer cell line (SUIT-2) producing carcinomembryonic antigen and carbohydrate antigen 19-9. Jpn J Cancer 78:54–62
Loor R, Kuriyama M, Bodziak MLM, Inaji H, Douglass HO, Berjian R, Nicorai JJ, Tytgat GN, Chu TM (1984) Simultaneous evaluation of a pancreas-specific antigen and a pancreatic cancer associated antigen in pancreatic carcinoma. Cancer Res 44:3604–3607
Magnani JL, Brockhaus M, Smith KF, Ginsburg V, Blaszczyk M, Mitchell KF, Steplewski Z, Koprowski H (1981) A monosialo-ganglioside is a monoclonal antibody-defined antigen of colon carcinoma. Science 212:55–56
Oriol R, Pendu JL, Mollicone R (1986) Genetics of ABO, H, Lewis, x and related antigens. Vox Sang 51:161–171
Pour PM, Takasaki H, Büchler M (1987a) Immunologic aspects of acute and chronic pancreatitis. Verh Dtsch Ges Pathol 71:255–265
Pour PM, Uchida E, Steplewski Z, Mroczek E, Büchler M, Beger HG (1987b) Immunologic aspects of acute pancreatitis. In: Beger HG, Büchler M (eds) Acute pancreatitis. Springer, Berlin Heidelberg New York, pp 41–44
Rouger P, Goossens D, Gane P, Salmon C (1981) Distribution of blood group antigens in adult pancreas. Tissue Antigens 18:51–55
Sakamoto K, Haga Y, Yoshimura R, Egami H, Yokoyama Y, Akagi M (1987) Comparative effec tiveness of the tumour diagnostics, CA19-9, CA125 and carcinoembrionic antigen in patients with diseases of the digestive system. Gut 28:323–329
Tempero MA, Uchida E, Takasaki H, Burnett DA, Steplewski Z, Pour PM (1987) Relationship of carbohydrate antigen 19-9 and Lewis antigens in pancreatic cancer. Cancer Res 47:5501–5503
Uchida E, Steplewski Z, Mrozczeck E, Buchler M, Burnett D, Pour PM (1986) Presence of two distinct acinar cell populations in human pancreas based on their antigenicity. Int J Pancreatol 1:213–225
Uchida E, Tempero MA, Burnett DA, Steplewski Z, Pour PM (1987) Correlative studies on antigenicity of pancreatic cancer and blood group types. Cancer Detect Prev [Suppl] 1:145–148
Yoshimura R, Sakamoto K, Egami H, Akagi M (1988) Immunohistochemistry of human gastrointestinal cancer-associated antigen detected by a monoclonal antibody PA8-15. Jpn J Cancer Res (Gann) 79:255–263
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This study was supported, in part, by core grant CA36727 from the NCI/NIH and SIG-16 from the American Cancer Society.
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Egami, H., Sakamoto, K., Yoshimura, R. et al. Comparative studies on the expression of gastrointestinal-cancer-associated antigen, PA8-15, CA19-9 and the blood-group antigens in non-malignant and malignant human pancreatic tissues. J Cancer Res Clin Oncol 116, 365–371 (1990). https://doi.org/10.1007/BF01612919
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DOI: https://doi.org/10.1007/BF01612919