Summary
We used the nude mouse tumour xenograft model to study the pathogenesis of mucosa alterations in the large bowel surrounding a carcinoma. In mouse colonic mucosa overlying HT-29 colonic carcinoma xenografts in the caecum, the crypts were elongated in comparison with those in distant mucosa and also frequently showed a shift towards sialomucin production. These features, which are comparable with socalled transitional mucosa (TM) in man, were absent in control animals inoculated with Indian Ink instead of HT-29 cells. Although the localization of the proliferative cell compartment in mouse colonic mucosa overlying HT-29 xenografts appeared to be confined to the lower half of the crypt as in normal mucosa, the relative length of the DNA synthesizing cell compartment along the crypts was slightly elongated. These data strongly suggest that TM should be regarded as a secondary phenomenon rather than a premalignant change in large intestinal epithelium and that higher proliferative activity of epithelial cells contributes little to the elongation of crypts in TM.
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Assoian RK, Frolik CA, Roberts AB, Miller DM, Sporn MB (1984) Transforming growth factor-controls receptor levels for epidermal growth factor in NRK fibroblasts. Cell 36:35–41
Coffey RJ, Shipley GD, Moses HL (1986) Production of transforming grwoth factors by human colon cancer lines. Cancer Res 46:1164–1169
Filipe MI (1969) Value of histochemical reactions for mucosubstances in the diagnosis of certain pathological conditions of the colon and rectum. Gut 10:577–586
Filipe MI (1975) Mucous secretion in rat colonic mucosa during carcinogenesis induced by dimethylhydrazine. A morphological and histochemical study. Br J Cancer 32:60–77
Franzin G, Grigioni WF, Dina R, Scarpa A, Zamboni G (1983) Mucin secretion and morphological changes of the mucosa in non-neoplastic diseases of the colon. Histopathology 7:707–718
Greaves P, Filipe MI, Branfoot AC (1980) Transitional mucosa and survival in human colorectal cancer. Cancer 46:764–770
Isaacson P, Attwood PRA (1979) Failure to demonstrate specificity of the morphological and histochemical changes in mucosa adjacent to colonic carcinoma (transitional mucosa). J Clin Pathol 32:214–218
Lanza G, Altavilla G, Gavazzini L, Negrini R (1985) Colonic mucosa adjacent to adenomas and hyperplastic polyps — a morphological and histochemical study. Histopathology 9:857–873
Lapertosa G, Fulcheri E, Acquarone M, Filipe MI (1984) Mucin prolifes in the mucosa adjacent to large bowel non-adenocarcinoma neoplasia. Histopathology 8:805–811
Lev R, Lance P, Camara P (1985) Histochemical and morphologic studies of mucosa bordering rectosigmoid carcinoma: comparisons with normal diseased, and malignant colonic epithelium. Hum Pathol 16:151–161
Lipkin M (1974) Phase 1 and phase 2 proliferative lesions of colonic epithelial cells in diseases leading to colonic cancer. Cancer 34:878–888
Listinsky CM, Riddell RH (1981) Patterns of mucin secretion of neoplastic and non-neoplastic diseases of the colon. Hum Pathol 12:923–929
Marcheggiano A, Iannoni C, Pallone F, Frieri G, Gallucci M, Caprill R (1983) Abnormal patterns of colonic mucin secretion after ureterosigmoidostomy. Hum Pathol 15:647–650
Marks ME, Brattain MG (1984) Induction of plasma membrane alterations in AKR-2B mouse embryo fibroblasts by endogenous growth factors from malignant human cells. Int J Cancer 33:139–146
Penelope AD, Filipe MI (1976) An ultrastructural and histochemical study of the mucous membrane adjacent to and remote from carcinoma. Cancer 37:2388–2398
Schutte B, Reynders MMJ, Bosman FT, Blijham GH (1987) Studies with antibromodeoxyuridine antibodies. II. Stimultaneous immunocytochemical detection of antigen expression and DNA synthesis by in vivo labeling of mouse intestinal mucosa. J Histochem Cytochem 35:371–374
Spicer SS (1965) Diamine methods for differentiating mucosubstances histochemically. J Histochem Cytochem 13:211–234
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Sekikawa, K., Arends, J.W., Verstijnen, K. et al. Colonic mucosal changes in nude mice associated with orthotopic xenografts of human colon cancer cells. Vichows Archiv A Pathol Anat 416, 393–396 (1990). https://doi.org/10.1007/BF01605143
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DOI: https://doi.org/10.1007/BF01605143