Abstract
Changes in the internal structure ofSaccharomyces cerevisiae cells and accumulation of proteins and nucleic components in extracellular fluid as decomposition products were studied in the 40–75°C temperature range under the effect of various membranotrophic additives. Autolysis was shown to be a two-step process: The first step consists of the restructuring of cell endostructures and the activation of lytic enzymes, which is accompanied by reduction of cell volume and system viscosity; the second step directly follows the first step and consists of hydrolysis of cell components and release of hydrolysis products into extracellular space. Duration of the first step depends on the temperature and the plasmolyzer. Hydrophilic additives (ethanol, ethyl acetate) were most effective during the first step at 60–65°C, whereas hydrophobic additives (lecithin, lauric acid) were most effective at 55°C. In the second step, the temperature optimum of protease activity in the control (without additives) was 60°C, that of nuclease activity was 70°C. Additives reduce the temperature optimum of endoenzymatic activity. Cell morphology was studied at various stages of autolysis by electron and phase-contrast microscopy.
Similar content being viewed by others
Literature Cited
Arima, K., Uozumi, T., Takahashi, M. 1965. Studies on the autolysis ofAspergillus oryzae. I. Condition of autolysis. Agricultural and Biological Chemistry29:1033–1041.
Belikov, V. M., Latov, V. K., Tsyriapkin, V. A., Sergeev, V. A. 1976. Yeast's biomass as a source of amino acids [In Russian]. Microbiologicheskay Promyshlennost [Microbiological Industry]3:1–6.
Bersin, T. 1951. Kurzes Lehrbuch der Enzymologie. Leipzig: Geest und Portig.
Bligh, E. G., Dyer, W. J. 1959. A rapid method of total lipid extraction and purification. Canadian Journal of Biochemistry and Physiology37:911–917.
Dreywood, R. 1946. Qualitative test for carbohydrate material. Industrial and Engineering Chemistry18:499.
Fields, R. 1971. The measurement of amino groups in proteins and peptides. Biochemical Journal124:581–590.
Iten, W., Matile, P. 1970. Role of chitinase and other lysosomal enzymes ofCoprinus lagopus in the autolysis of fruiting bodies. Journal of General Microbiology61:301–309.
Johnson, J. C. 1977. Yeast for food and other purposes. Park Ridge, New Jersey: Noyes Data Corporation.
Matile, P. 1969. Zellphysiologie. Reaktionsträume der Pflanzenzelle: Lysosomen und Peroxysomen. Fortschritte der Botanik31:64–75.
Moor, H. 1964. Die Gefrier-Fixation lebender Zellen und ihre Anwendung in der Elektronenmikroskopie. Zeitschrift für Zellforschung und Mikroskopische Anatomie62:546–580.
Okunaki, K. 1963. Analytical methods of protein chemistry, p. 60. In: Orekhovitch, V. N. (ed.), vol. 60. Moscow: Foreign Literature.
Pokrowsky, A. A., Toutelyan, V. A. 1977. Lysosomes. Moscow: Nauka.
Shapot, V. S. 1968. Nucleases. Moscow: Medicina.
Spirin, A. S. 1958. Spectrophotometric determination of total nucleic acid content [In Russian]. Biochemia [Biochemistry]23:617–622.
Trevelyan, W. E. 1976. Induction of autolytic breakdown of RNA in yeast by addition of ethanol and by drying/rehydration. Journal of the Science of Food and Agriculture27:570–588.
Vasilieva, R. P., Evtichov, P. N., Bogorad, G. V. 1976. Relationship between proteinase activities obtained by two different methods [In Russian]. Microbiologicheskay Promyshlennost [Microbiological Industry]4:23–26.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Babayan, T.L., Bezrukov, M.G., Latov, V.K. et al. Induced autolysis ofSaccharomyces cerevisiae: Morphological effects, rheological effects, and dynamics of accumulation of extracellular hydrolysis products. Current Microbiology 5, 163–168 (1981). https://doi.org/10.1007/BF01578522
Issue Date:
DOI: https://doi.org/10.1007/BF01578522