Abstract
The Daudi cell line, established from a Burkitt lymphoma, has recently been found to be HLA- andΒ 2-microglobulin-negative, although it expresses B lymphocyte alloantigens. This report is concerned with the reexpression of HLA-A10, B38, and B17 on the Daudi cell, after cell fusion with another human cell line (Raji) or with mouse fibroblasts. In the latter fusion, the same HLA specificities are re-expressed, but not humanΒ 2-microglobulin while mouseΒ 2-microglobulin andH-2 could be detected. No such reexpression was observed when Daudi was fused with the F9 mouse teratocarcinoma, which lacks mouseΒ 2-m andH-2. No HLA activity (alloantigenic and xenogenic activity) was detected in the membrane or cytoplasm of Daudi, using salt extraction and sonication. Therefore we postulate thatΒ 2-microglobulin could be necessary for the expression and possible synthesis of the HLA antigen.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Appella, E., Tanigaki, N., Watanori, T., and Plessman, D.: Partial amino acid sequence of mouse B2-microglobulin.Biol. Biochem. Acta 70:425–430, 1976
Artzt, K., Dubois, P., Bennett, D., Condamine, H., Babinet, C., and Jacob, F.: Surface antigen common to mouse cleavage embryos and primitive teratocarcinoma in culture.Proc. Natl. Acad. Sci. U.S.A. 70:2988–2992, 1973
Bernstine, E.G., Hooper, M.L., Grandchamp, S., and Ephrussi, B.: Alkaline phosphatase activity in mouse teratoma.Proc. Natl. Acad. Sci. U.S.A. 70:3899–3903, 1973
Clements, G., Klein, G., Zeuthen, J., and Povey, S.: The selection of somatic cell hybrids between human lymphoma cell lines.Somatic Cell Genet., 2:309–324, 1976
Cresswell, P., Springer, T., Strominger, J.L., Turner, M.J., Grey, H.M., and Kubo, R.T.: Immunological identity of the small subunit of HLA antigens and B2 microglobulin and its turnover on the cell membrane.Proc. Natl. Acad. Sci., U.S.A. 71:2123–2127, 1974
Dubois, P., Fellous, M., Gachelin, G., Jacob, F., Kemler, R., Pressman, D., and Tanigaki, N.: Absence of a serologically detectable association of human and murine B2 microglobulin with the F9 antigen.Transplantation 22:467–473, 1976
Evrin, P.E. and Nilsson, K.: B2-microglobulin production in vitro by human hematopoietic, mesenchymal and epithelial cells.J. Immunol. 112:137–144, 1974
Fellous, M., Mortchelewicz, F., Kamoun, M., and Dausset, J.: The use of a lymphoid cell line to define new B lymphocyte antigens, probably controlled by the MHC region.In F. Kissmeyer-Nielsen (ed.):Histocompatibility Testing, pp. 708–711, Munksgaard, Copenhagen, 1975
Fellous, M., Colle, A., and Tonnelle, C.: The expression of human B2m microglobulin on human spermatozoa.Eur. J. Immunol. 6:21–24, 1976
Fellous, M., Hors, M.C., Mahouy, G., and Fridman, W.H.: The expression and relation of HLA, Β2 microglobulin and receptor for marmoset red blood cells on man/mouse hybrid cells.Eur. J. Immunol., 7:22–26, 1977
Ferrone, S., Pellegrino, M.A., and Reisfeld, R.A.: A rapid method for direct HLA typing of cultured lymphoid cells.J. Immunol. 107:613–615, 1971
Goodfellow, P.N., Jones, E.A., van Heyningen, V., Solomon, E., Bobrow, M., Miggiano, V., and Bodmer, W.P.: TheΒ2-microglobulin gene is on chromosome 15 and not in the HLA region.Nature 254:267–269, 1975
Jones, E.A., Goodfellow, P.N., Bodmer, J.G., and Bodmer, W.F.: Serological identification of HLA linked human Ia typed antigens.Nature 256:650–653, 1975
Klein, E., Klein, G., Nadkarni, J.S., Nadkarni, J.J., Wigzell, H., and Clifford, P.: Surface IgM kappa specificity on a Burkitt lymphoma cell in vivo and in derived culture lines.Cancer Res. 28:1300–1310, 1968
Klein, G., Terasaki, P., Billing, R., Honig, R., Jondal, M., Rosen, A., Zeuthen, J., and Clements, G.: Somatic cell hybrids between human lymphoma cell lines. III. Surface markers.Int. J. Cancer 19:66–76, 1977
Nakamuro, K., Tanigaki, N., and Pressman, D.: Isolation of HLA 33,000 dalton fragments carrying high HLA alloantigenic activity.Transplantation 19:431–437, 1975
Ostberg, L., Rask, L., Nilsson, K., and Peterson, P.A.: Human Ia type alloantigens: methods of detection, aspect of chemistry and biology, markers for disease states.Eur. J. Immunol. 5:462–468, 1975
Reisfeld, R.A., Pellegrino, M.A., and Kahan, M.D.: Salt-extraction of soluble HLA antigens.Science 172:1134–1136, 1971
Reisfeld, R.A., Sevies, E.D., Pellegrino, M.A., Ferrone, S., and Poulik, M.D.: Association of HLA antigens andΒ 2-microglobulin of the cellular level.Immunogenetics 2:184–197, 1975
Shulman, N.R., Marder, V.J., Hiller, M.C., and Collier, E.M.: Platelet and leukocyte isoantigens and their antibodies: serologic, physiologic and clinical studies.Prog. Hemat. 4:222–224, 1964
Someren, H., Westerveld, A., Hagemeijer, A., Meera-Khan, P., and Zaalberg, O.: Human antigen and enzyme markers in man-Chinese hamster somatic cell hybrids evidence for synteny between the HLA, PGm3, ME1, and IPO-B loci.Proc. Natl. Acad. Sci. U.S.A. 71:962–965, 1974
Ting, A., Mickey, M.R., and Terasaki, P.I.: B-lymphocyte alloantigens in Caucasians.J. Exp.
Wernet, P.: Human Ia type alloantigens: methods of detection, aspect of chemistry and biology, markers for disease states.Transplant. Rev. 30:271–298, 1976
Zeuthen, J., Friedrich, U., Rosen, A., and Klein, E.: Structural abnormalities in chromosome 15 in cell lines with reduced expression of beta-2-microglobulin.Immunogenetics, in press (1977)
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Fellous, M., Kamoun, M., Wiels, J. et al. Induction of HLA expression in Daudi cells after cell fusion. Immunogenetics 5, 423–436 (1977). https://doi.org/10.1007/BF01570501
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01570501