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Interleukin-1 treatment increases neutrophils but not antioxidant enzyme activity or resistance to ischemia-reperfusion injury in rat kidneys

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Abstract

Hearts from rats treated with interleukin-1 (IL-1) intraperitoneally developed a rapid (6 h after IL-1), transient increase in neutrophils, tissue hydrogen peroxide (H2O2), and oxidized glutathione (GSSG) levels, and a subsequent (36 h after IL-1) increase in myocardial glucose-6-phosphate dehydrogenase (G6PD) activity and tolerance to ischemia-reperfusion. In the present investigation, we found that rats treated similarly with IL-1 had increased numbers of neutrophils in their kidneys, which were comparable to myocardial neutrophil increases, but did not develop increased renal tissue H2O2 or GSSG levels acutely (6 h after IL-1) or increased G6PD activity or resistance to ischemia-reperfusion injury later (36 h after IL-1). Our findings indicate that IL-1 treatment increased neutrophil accumulation in rat kidneys but did not increase oxidative stress, antioxidant enzyme activity, or resistance to ischemia-reperfusion injury. We conclude that organ-to-organ differences exist with respect to IL-1-induced tolerance.

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Authors and Affiliations

  1. Webb-Waring Institute for Biomedical Research Department of Medicine at Denver General Hospital, University of Colorado Health Sciences Center, Denver, Colorado

    David M. Guidot, Stuart L. Linas, Michael J. Repine, Paul F. Shanley, Halee S. Fisher & John E. Repine

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  1. David M. Guidot
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  2. Stuart L. Linas
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  3. Michael J. Repine
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  4. Paul F. Shanley
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  5. Halee S. Fisher
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  6. John E. Repine
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Guidot, D.M., Linas, S.L., Repine, M.J. et al. Interleukin-1 treatment increases neutrophils but not antioxidant enzyme activity or resistance to ischemia-reperfusion injury in rat kidneys. Inflammation 18, 537–545 (1994). https://doi.org/10.1007/BF01560700

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  • DOI: https://doi.org/10.1007/BF01560700

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