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Ultrastructural and biochemical studies of ischemic preconditioning using an adenosine receptor blocker and an ATP-sensitive K channel opener

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Abstract

The effect of preconditioning (PC) on acute ischemic myocardial injury was investigated in an openchest dog model. Preconditioned dogs received four 5-min occlusions of the left anterior descending coronary artery (LAD), each separated by 10 min of reperfusion. Four groups were used to assess the effect: non-PC group (G-1), PC group (G-2), 8-phenyltheophylline-(adenosine receptor blocker) infused PC group (G-3), and nicorandil- (ATP-sensitive K-channel opener) infused PC group (G-4). The LAD was occluded for 60 min, followed by 60 min of reperfusion in all dogs. The rate of ultrastructural myocardial severe injury was 26% in G-1, 0% in G-2, 5% in G-3, and 0% in G-4. Biochemical analayses also indicated higher values of myocardial contractile function in G-2 and G-4 than G-1 and G-3. These data suggest that the adenosine receptor and K channel may play a key role in PC.

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Authors and Affiliations

  1. Third Department of Internal Medicine, Showa University School of Medicine, 1-5-8 Hatanodai, Shinagawa-ku, 142, Tokyo, Japan

    Masaki Nakatani, Haruhiko Ishioka, Shinji Koba, Ryuji Ueda, Hiroshi Suzuki, Hirohisa Arata, Tohru Kitsu, Eiichi Geshi & Takashi Katagiri

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  1. Masaki Nakatani
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  2. Haruhiko Ishioka
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  3. Shinji Koba
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  4. Ryuji Ueda
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  5. Hiroshi Suzuki
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  6. Hirohisa Arata
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  7. Tohru Kitsu
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  8. Eiichi Geshi
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  9. Takashi Katagiri
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Nakatani, M., Ishioka, H., Koba, S. et al. Ultrastructural and biochemical studies of ischemic preconditioning using an adenosine receptor blocker and an ATP-sensitive K channel opener. Med Electron Microsc 30, 63–69 (1997). https://doi.org/10.1007/BF01545083

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  • DOI: https://doi.org/10.1007/BF01545083

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