Abstract
A simple procedure has been worked out to obtain UV-sensitive mutants of Chinese hamster ovary (CHO) cells. In this procedure, conventional mutagenesis is followed by BrdU-light treatment to enrich the population for UV-sensitive cells. Colonies that are allowed to form subsequently are duplicated by replica plating and screened on the master plate for their UV sensitivity and their capacity to carry out UV-induced DNA repair synthesis. Putative mutants are isolated from the replica. With this combination of methods, we succeeded in isolating CHO mutants with an 85–95% reduced level of UV-induced DNA synthesis in combination with an increased UV sensitivity.
Similar content being viewed by others
Literature cited
Bootsma, D. (1978). InDNA Repair Mechanisms, (eds.) Hanawalt, P.C., Friedberg, E.C., and Fox, C.F. Academic Press, New York, pp. 589–601.
Kraemer, K.H. (1980).Clin. Dermatol. 4:1–33.
Sinclair, W.K. (1964).Radiat. Res. 21:584–611.
Todd, P.W., and Hellewel, A.B. (1969).Mutat. Res. 7:129–132.
Isomura, K., Nikaido, O., Horikawa, M., and Sugahara, T. (1973).Radiat. Res. 53:143–152.
Kuroki, T., and Miyashita, S.Y. (1977).J. Cell Physiol. 90:79–90.
Stamato, T.D., and Waldren, C.A. (1977).Somat. Cell Genet. 3:431–440.
Schultz, R., Chang, C.C., and Trosko, J.E. (1978).Proc. of Am. Assoc. Cancer Res. Am. Soc. Clin. Oncol. 19:28 (abstract).
Rosenstein, B., and Ohlsson-Wilhelm, B.M. (1979).Somat. Cell Genet. 5:117–128.
Sato, K., and Hieda, N. (1979).Int. J. Radiat. Biol. 35:83–87.
Sato, K., and Hieda, N. (1979).Radiat. Res. 78:167–171.
Shiomi, T., and Sato, K. (1979).Somat. Cell Genet. 5:193–201.
Busch, D.B., Cleaver, J.E., and Glaser, D.A. (1980).Somat. Cell Genet. 6:407–418.
Thompson, L.H., Rubin, J.S., Cleaver, J.E., Witmore, G.F., and Brookman, W. (1980).Somat. Cell Genet. 6:391–405.
Chang, C.C., Boesi, J.A., Warren, S.T., Sabourin, S.T., Sabourin, C.L.K., Liu, P.K., Glatzer, L., and Trosko, J.E. (1981).Somat. Cell Genet. 7:235–253.
Schultz, R.A., Trosko, J.E., and Chang, C.C. (1981).Environ. Mutagenesis 3:53–64.
Schultz, R.A., Chang, C.C, and Trosko, J.E. (1981).Environ. Mutagenesis 3:141–150.
Kao, F.T., and Puck, T.T. (1968).Proc. Natl. Acad. Sci. U.S.A. 60:1275–1282.
Russel, W.C., Newman, C., and Williamson, D.H. (1975).Nature 253:461–462.
Esko, J.D., and Raetz, C.R.H. (1978).Proc. Natl. Acad. Sci. U.S.A. 75:1190–1193.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Stefanini, M., Reuser, A. & Bootsma, D. Isolation of Chinese hamster ovary cells with reduced unscheduled DNA synthesis after UV irradiation. Somat Cell Mol Genet 8, 635–642 (1982). https://doi.org/10.1007/BF01542856
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01542856