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The independent expression of HLA andβ 2-microglobulin on human-mouse hybrids

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Somatic Cell Genetics

Abstract

Direct cytotoxicity and absorption analysis were used to detect HLA antigens on man/mouse somatic cell hybrids. Confirmation of the assignment of the Major Histocompatibility Complex to chromosome 6 was obtained. The same series of hybrids were used to study the interaction between HLA and β2m on the cell surface. Results obtained from a series of clones indicated that there was complete independence of expression of HLA and β2m. Human β2m was found to be unnecessary for the normal serological expression of HLA, suggesting that mouse β2m could possibly replace human β2m as a subunit of the HLA molecule. Evidence is presented demonstrating that this is in fact the case. Heteroantisera to human β2m and alloantiserum to HLA-A2 were used, together with complement, to selectively remove the chromosome coding for β2m and HLA from a hybrid clone. Manipulation of the karyotype in this way further confirmed the independence of HLA and β2m on somatic cell hybrids.

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Authors and Affiliations

  1. Genetics Laboratory, Department of Biochemistry, University of Oxford, Oxford, England

    E. A. Jones & W. F. Bodmer

  2. Division of Immunology, Stanford University, School of Medicine, Stanford, California

    P. N. Goodfellow

  3. Department of Human Genetics, School of Medicine, University of Pennsylvania, Philadelphia, Pennsylvania

    R. H. Kennett

Authors
  1. E. A. Jones
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  2. P. N. Goodfellow
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  3. R. H. Kennett
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  4. W. F. Bodmer
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Jones, E.A., Goodfellow, P.N., Kennett, R.H. et al. The independent expression of HLA andβ 2-microglobulin on human-mouse hybrids. Somat Cell Mol Genet 2, 483–496 (1976). https://doi.org/10.1007/BF01542686

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  • DOI: https://doi.org/10.1007/BF01542686

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