Abstract
Hybridoma cells which secrete colorectal carcinoma-specific antibodies have been produced and used to study the antigenic structure of these tumor cells. Nineteen antibodies have been studied in detail, and 15 of these are colorectal carcinoma specific. Only two antibodies reactive with carcinoembryonic antigen (CEA) have been discovered and five other antibodies that react with distinct epitopes on the cell surface have been defined. Several antigens with distinct molecular characteristics have been shown to exist by use of hybridoma antibodies. Six hybridoma antibodies have been shown to mediate antibody-dependent cell-mediated cytotoxicity (ADCC).
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One of us (Hilary Koprowski) had the privilege and pleasure of collaborating with Boris Ephrussi during the latter's stay in the United States. In collaboration with Vittorio Defendi, we studied the expression of polyoma-induced antigen in the second somatic cell hybrid ever made (1) and of tumorigenicity in other hybrids between polyoma-transformed and normal mouse cells (2). It has been both astonishing and amazing to watch the progress made during the subsequent two decades in the field of somatic cell hybridization, and we deeply regret that Boris Ephrussi, who contributed so much to this progress, can no longer share our enthusiasm.
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Koprowski, H., Steplewski, Z., Mitchell, K. et al. Colorectal carcinoma antigens detected by hybridoma antibodies. Somat Cell Mol Genet 5, 957–971 (1979). https://doi.org/10.1007/BF01542654
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DOI: https://doi.org/10.1007/BF01542654