Abstract
Human fibroblasts containing a translocation between the X chromosome and chromosome 15 were fused with the 6-thioguanine-resistant mouse cell line, IR. Resulting hybrids, selected in HAT medium, retained the X/15 chromosome. Hybrids which were counterselected in 6-thioguanine lost this chromosome. The X-linked markers glucose-6-phosphate dehydrogenase (G6PD), phosphoglycerate kinase (PGK), and hypoxanthine phosphoribosyl transferase (HPRT), and the non-X-linked markers pyruvate kinase (PKM2) mannose phosphate isomerase (MPI), N-acetyl hexosaminidase A (HEXA) and β2-microglubulin (β2-m) all segregated in concordance with the X/15 translocation chromosome. The latter markers have been assigned to chromosome 15. Selection against the X/15 chromosome was done using antihuman β2-m serum. Electrophoretic and immunochemical analyses of the N-acetyl hexosaminidases A and B in these hybrids were performed.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
Literature cited
Weiss, M.C., and Green H. (1967).Proc. Natl. Acad. Sci. 58:1104–1111.
Littlefield, J.W. (1964).Cold Spring Harbor Symp. Quant. Biol. 29:161–166.
Solomon, E., Goodfellow, P., and Bobrow, M. (1975).Heredity 35:145.
Nabholz, M., Miggiano, V., and Bodmer, W.F. (1969).Nature 223:358–363.
Pontecorvo, G. (1971).Nature 230:367–369.
Bobrow, M., and Cross, J. (1974).Nature 251:77–79.
van Heyningen, V., Bobrow, M., Bodmer, W.F., Gardner, S.E., Povey, S., and Hopkinson, D.A. (1975).Ann. Hum. Genet. 38:295–303.
Watson, B., Gormley, I.P., Gardiner, S.E., Evans, H.J., and Harris, H. (1972).Exp. Cell Res. 75:401–409.
Swallow, D.M., Stokes, D.C., Corney, G., and Harris, H. (1974).Ann. Hum. Genet.,37:287–302.
Giblett, E.F. (1969).Genetic Markers in Human Blood, Blaekwell Scientific Publications, p. 169.
Goodfellow, P.N., Jones, E.A., van Heyningen, V., Solomon, E., Bobrow, M., Miggiano, V., and Bodmer, W.F. (1975).Nature 254:267–269.
Nilsson, K., Evrin, P.E., Welsh, K.I. (1974).Transplant. Rev. 21:53–84.
Therman, E., and Patan, K. (1974).Humangenetik 25:1–16.
Rotterdam Conference (1974).2nd International Workshop on Human Gene Mapping, The National Foundation, New York.
Transplantation Reviews (1974).21.
Kusano, T., Long, C., and Green, H. (1971).Proc. Natl. Acad. Sci. U.S.A. 68:82–86.
Tischfield, J.A. and Ruddle, F.H. (1974)Proc. Natl. Acad. Sci. U.S.A. 71:45–49.
Baker, R.M., Brunette, D.M., Mankovitz, R., Thompson, L.H., Whitmore, G.F., Siminovitch, L., and Till, J.E. (1974).Cell 1:9–21.
Creagan, R.P., Chen, S., and Ruddle, F.H. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:2237–2241.
Buck, D., and Bodmer, W.F. (1975).2nd International Workshop on Human Gene Mapping, The National Foundation, New York, pp. 87–89.
Puck, T.T., Wuthier, P., Jones, C., and Kao, F. (1971).Proc. Natl. Acad. Sci. 68:3102–3106.
Lalley, P.A., Rattazzi, M.C., and Shows, T.B. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:1569–1573.
Nguyen Van Cong, N., Rebourcet, R., Weil, D., Billardon, C., Blanchard, M.G., Finaz, C., and Frezal, J. (1974).C.R. Acad. Sci. Paris 278:1761–1764.
Gilbert, F., Kucherlapati, R., Creagan, R.P., Murane, M.J., Darlington, G.J., and Ruddle, F.H. (1975).Proc. Natl. Acad. Sci. U.S.A. 72:263–267.
Nguyen Van Cong, Weil, D., Rebourcet, R., and Frezal, J. (1975).Ann. Hum. Genet. 39:111–123.
van Someren, H., and van Henegouwan, H.B. (1973).Humangenetik 18:1–4.
Lamm, L.V., Kissmeyer-Nielsen, F., Svejgaard, A., Bruun Peterson, G., Thorsby, E., Mayr, W., and Högman, C. (1972).Tissue Antigens 2:205–214.
van Someren, H., Westerveld, A., Hagemeyer, A., Mees, J.R., and Meera Khan, P. (1974).Proc. Natl. Acad. Sci. U.S.A. 71:962–965.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Solomon, E., Bobrow, M., Goodfellow, P.N. et al. Human gene mapping using an X/autosome translocation. Somat Cell Mol Genet 2, 125–140 (1976). https://doi.org/10.1007/BF01542626
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01542626