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Cowpea aphid borne mosaic virus-Morocco and South AfricanPassiflora virus are strains of the same potyvirus

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Summary

High performance liquid chromatography (HPLC) profiles of tryptic peptides and partial amino acid sequence analysis have been employed to establish the taxonomic status of the Moroccan isolate of cowpea aphid-borne mosaic virus (CABMV). Some previous reports have suggested CABMV to be very closely related to blackeye cowpea mosaic virus (B1CMV) while other reports have concluded that this relationship is distant. In this report a tryptic digest of the coat protein of CABMV-Morocco was compared with those of the coat proteins of B1CMV-Type, B1CMV-W, the mild mottle strain of peanut stripe virus (PStV-MM) and the NY15 strain of bean common mosaic virus (BCMV-NY15), all of which are now recognised as strains of BCMV. The comparisons also included the NL-3 strain of bean necrosis mosaic virus (BNMV-NL3), which had previously been classified as a strain of BCMV. The HPLC peptide profiles indicated that CABMV-Morocco was distinct from BCMV and BNMV. Amino acid sequence analysis of peptides accounting for more than half of the coat protein confirmed that CABMV-Morocco was not a strain of BNMV or BCMV but was a distinct member of the BCMV subset of viruses that previously has been shown to include BCMV, BNMV, soybean mosaic virus, zucchini yellow mosaic virus, passionfruit woodiness virus and South AfricanPassiflora virus (SAPV). Comparison of the partial sequence data with these and other published sequences revealed that the coat protein of CABMV-Morocco is very similar to that of SAPV suggesting that they are strains of the same virus. Since CABMV was described over 25 years earlier than SAPV, the name CABMV should take precedence and SAPV should be renamed CABMV-SAP, the South AfricanPassiflora strain of CABMV.

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Authors and Affiliations

  1. CSIRO Division of Biomolecular Engineering, Parkville, Australia

    N. M. McKern, P. M. Strike, D. D. Shukla & C. W. Ward

  2. Department of Plant Pathology, North Carolina State University, Raleigh, NC, USA

    O. W. Barnett

  3. Department of Virology, Wageningen Agricultural University, Wageningen, The Netherlands

    J. Dijkstra

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  1. N. M. McKern
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  2. P. M. Strike
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  3. O. W. Barnett
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  4. J. Dijkstra
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  5. D. D. Shukla
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  6. C. W. Ward
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McKern, N.M., Strike, P.M., Barnett, O.W. et al. Cowpea aphid borne mosaic virus-Morocco and South AfricanPassiflora virus are strains of the same potyvirus. Archives of Virology 136, 207–217 (1994). https://doi.org/10.1007/BF01538830

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  • DOI: https://doi.org/10.1007/BF01538830

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