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Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation

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Somatic Cell Genetics

Abstract

We have transferred DNA to Chinese hamster ovary (CHO) cells by DNA-mediated transformation. CHO tk− cells were transformed with the cloned gene for herpes simplex virus thymidine kinase (HSV-tk) and were found to have a 50-fold lower frequency of transformation than mouse Ltk− cells at the same DNA dosage. By altering the amount of tk gene and carrier DNA present, frequencies of up to 5 × 10−5 were obtained. CHO HSV- tk+ transformants were very stable, and in several clones the HSV-tk gene copies integrated in higher-molecular-weight DNA. These cells also exhibited cotransformation for unselected markers. CHO lines were also transformed at a frequency of 10−4 with the bacterial gene Ecogpt in a SV40-pBR322 vector. CHO tk− cells could be transformed at a frequency of 10−7 with cellular DNA isolated from CHO tk+ cells. CHO cells offer a well-defined genetic system within which to transfer either cloned or whole cellular DNAs.

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Authors and Affiliations

  1. Laboratory of Molecular Biology, National Cancer Institute, 20205, Bethesda, Maryland

    Irene Abraham, Jaya Sivaswami Tyagi & Michael M. Gottesman

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  1. Irene Abraham
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  2. Jaya Sivaswami Tyagi
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  3. Michael M. Gottesman
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Abraham, I., Tyagi, J.S. & Gottesman, M.M. Transfer of genes to chinese hamster ovary cells by DNA-mediated transformation. Somat Cell Mol Genet 8, 23–39 (1982). https://doi.org/10.1007/BF01538648

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  • DOI: https://doi.org/10.1007/BF01538648

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