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Assay of growth factors for prostate epithelial cells

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Journal of tissue culture methods

Summary

The maintenance and proliferation of isolated prostate epithelial cells is androgen independent, but requires multiple other hormones and hormonelike growth factors. Methods are described for isolation and characterization of epithelial cells from normal rat prostate and the androgen-responsive transplantable Dunning R3327 rat tumor. Pure tumor-derived cell lines can be established by serial culture techniques. The normal primary and serially cultured cell lines are then used to assay growth factors. Cell proliferation is quantitated by computerized videometry.

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References

  1. Coffey, D. S.: Isaacs, J. T. Models for prostate cancer requirements for an idealized animal model of prostate cancer. In: Murphy, G. N., ed. New York: Alan R. Liss; 1980:379–391.

    Google Scholar 

  2. Crabb, J. W.; Armes, L. G.: Johnson, C.M., et al. The complete primary structure of prostatropin, a prostate epithelial cell growth factor. Biochemistry 25:4988–4933; 1986.

    PubMed  Google Scholar 

  3. Isaacs, J. T.; Weissman, R. M.; Coffey, D. S., et al. Concepts in prostatic cancer biology: Dunning R-3327H, HI, and AT tumors. In: Murphy, G. N., ed. Models for prostate cancer. New York: Alan R. Liss; 1982:311–323.

    Google Scholar 

  4. Lechner, J. F.; Babcock, M. S.; Marnell, M., et al. Normal human prostate epithelial cell cultures. Methods Cell Biol. 21B:195–225; 1980.

    PubMed  Google Scholar 

  5. McKeehan, W. L.; Adams, P. S.; Rosser, M. P. Direct mitogenic effects of insulin, epidermal growth factor, glucorticoid, choleratoxin, unknown pituitary factors and possibly prolactin, but not androgen on normal rat prostate epithelial cells in serum-free, primary cell culture. Cancer Res. 44:1988–2010; 1984.

    Google Scholar 

  6. McKeehan, W. L.; Adams, P. S.; Fast, D. Different hormonal requirements for androgen-independent growth of normal and tumor epithelial cells from rat prostate. In Vitro 23:147–152; 1987.

    Google Scholar 

  7. McKeehan, W. L.; McKeehan, K. A. Kinetic analysis of cell growth factor-nutrient interactions. Methods Enzymol. In press; 1987.

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Authors and Affiliations

  1. W. Alton Jones Cell Science Center, Inc., 10 Old Barn Road, 12946, Lake Placid, NY

    Wallace L. McKeehan & Pamela S. Adams

Authors
  1. Wallace L. McKeehan
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  2. Pamela S. Adams
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McKeehan, W.L., Adams, P.S. Assay of growth factors for prostate epithelial cells. Journal of Tissue Culture Methods 10, 151–154 (1986). https://doi.org/10.1007/BF01404605

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  • DOI: https://doi.org/10.1007/BF01404605

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