Summary
A technique for maintaining mammalian ovaries in organ culture is described. Ovaries of rats, mice, monkeys and humans have been maintained in a histologically normal condition for up to 20 days in culture, during which many were X-irradiated.
The doses of X-rays required to destroy all the primordial oocytes in organ cultures are similar to those reported forin vivo studies.
The human foetal ovary is resistant to X-irradiation. Some oocytes survive for 7 days following exposure to 4000 R in organ culture. The radiation sensitivity of the human ovary is discussed: it would appear that it is amongst the most resistant to oocyte-killing known.
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Paper read at the 6th Annual Meeting of the European Society for Radiobiology, Interlaken, 5.–8. June, 1968. Round Table: Radiation Effectsin vitro andin vivo. Correlations and Discrepancies.
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Baker, T.G., Neal, P. The effects of X-irradiation on mammalian oocytes in organ culture. Biophysik 6, 39–45 (1969). https://doi.org/10.1007/BF01403867
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DOI: https://doi.org/10.1007/BF01403867