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Analysis of temperature dependence of cytoplasmic streaming using tonoplast-free cells of Characeae

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Summary

The temperature dependence of cytoplasmic streaming in intact and tonoplast-free cells ofNitellopsis obtusa was studied using a cryomicroscope. The streaming velocity decreases linearly with decrease in the temperature in well-buffered tonoplast-free cells but non-linearly in some intact cells. These results suggest that low temperature causes a disturbance in the homeostasis of calcium and protons, which inhibit cytoplasmic streaming in intact cells.

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Abbreviations

ADP:

adenosine 5′-diphosphate

APW:

artificial pond water

ATP:

adenosine 5′-triphosphate

EGTA:

ethylene glycol-bis(β-aminoethyl ether)N,N′,N′-tetraacetic acid

HEPES:

N-(2-hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

PIPES:

piperazine-N, N′-bis(2-ethanesulfonic acid)

Tris:

tris(hydroxymethyl)aminoethane

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Authors and Affiliations

  1. The Institute of Low Temperature Science, Hokkaido University, Sapporo, Hokkaido

    S. Yoshida

  2. Department of Life Science, Faculty of Science, Himeji Institute of Technology, Harima Science Park City, 678-12, Hyogo, Japan

    T. Shimmen

Authors
  1. T. Shimmen
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  2. S. Yoshida
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Shimmen, T., Yoshida, S. Analysis of temperature dependence of cytoplasmic streaming using tonoplast-free cells of Characeae. Protoplasma 176, 174–177 (1993). https://doi.org/10.1007/BF01378954

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  • DOI: https://doi.org/10.1007/BF01378954

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