Abstract
Allele and phenotype frequencies for D1S80, D17S5 and ApoB were determined in a population sample of more than 200 unrelated persons from North Poland using the PCR method. For D1S80, D17S5 and ApoB 19, 13 and 21 alleles respectively were observed. No deviations from Hardy-Weinberg equilibrium were detected. All three systems have discrimination values above 92% and a cumulative discrimination index of 4.5 × 104.
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References
Allen RC, Graves G, Budowle B (1989) Polymerase chain reaction amplification products separated on rehydratable polyacrylamide gels and stained with silver. Biotechniques 7:736–741
Bassam BJ, Caetano-Anolles G, Gresshoff PM (1991) DNA amplification fingerprinting of bacteria. Anal Biochem 80:81–84
Boerwinkle E, Xiong W, Fourest E, Chan L (1989) Rapid typing of tandemly repeated hypervariable loci by the polymerase chain reaction: application of the apolipoprotein B 3′ hypervariable region. Proc Natl Acad Sci USA 86:212–216
Botstein D, White RL, Skolnick M, Davis RW (1980) Construction of a genetic linkage map in man using restriction fragment length polymorphism. Am J Hum Genet 32:182–190
Deka R, DeCroo S, Yu LM, Ferrell RE (1992) Variable number of tandem repeat (VNTR) polymorphism at locus D17S5 (D17S5) in four ethnically defined human populations. Hum Gent 90:86–91
Deka R, DeCroo S, Jin L, McGarvey ST, Rothhammer F, Ferrell RE, Chakraborty R (1994) Population genetic characteristics of the D1S80 locus in seven human populations. Hum Genet 94:252–258
Horn GT, Richards B, Klinger KW (1989) Amplification of a highly polymorphic VNTR segment by the polymerase chain reaction. Nucleic Acids Res 17:2140
Jones DA (1972) Blood samples: probability of discrimination. J Forensic Sci Soc 12:355–359
Kasai K, Nakamura Y, White R (1990) Amplification of highly polymorphic VNTR locus (pMCT 118) by the polymerase chain reaction (PCR) and its application to forensic science. J Forensic Sci 35:1196–1200
Kloosterman AD, Budowle B, Daselaar P (1993) PCR-amplification and detection of the human D1S80 VNTR locus. Int J Legal Med 105:257–264
Lahiri DK, Bye S, Nurnberger JI, Hodes ME; Crisp M (1992) A non-organic and non-enzymatic extraction method gives higher yields of genomic DNA from whole blood samples than do nine other methods tested. J Biochem Biophys Methods 25:193–205
Rand S, Puers C, Skowasch K, Wiegand P, Budowle B, Brinkmann B (1992) Population genetics and forensic efficiency data of 4 AMPFLPs. Int J Legal Med 104:329–333
Woller J, Furedi S, Padar Z (1995) AMPFLP analysis of the VNTR loci D1S80 and ApoB in Hungary. Int J Legal Med 107:273–274
Wong Z, Wilson V, Patel I, Povey S, Jeffreys AJ (1987) Characterization of a panel of highly variable minisatellites clones from human DNA. Ann Hum Genet 51:269–288
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Dedicated to Prof. Dr. J. Gerchow on the occasion of his 75th anniversary
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Pawlowski, R., Paszkowska, R., Hauser, R. et al. Population studies of three AMPFLPs systems in a North Polish population. Int J Leg Med 109, 155–156 (1996). https://doi.org/10.1007/BF01369678
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DOI: https://doi.org/10.1007/BF01369678