Summary
The V protein of Newcastle disease virus (NDV) is produced by the insertion of a single nontemplated G residue at a specific point during transcription of the phosphoprotein (P) gene, accessing a new reading frame upon translation. The V protein, in common with its counterpart in other paramyxoviruses contains a highly cysteine rich motif near the carboxyl terminus, suggestive of a zinc-binding domain. By constructingE. coli overexpression plasmids for the NDV P and V proteins, and monitoring the binding of65ZnCl2 to proteins electroblotted onto nitrocellulose membranes, we have demonstrated that the V protein strongly binds zinc.
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Steward, M., Samson, A.C.R., Errington, W. et al. The Newcastle disease virus V protein binds zinc. Archives of Virology 140, 1321–1328 (1995). https://doi.org/10.1007/BF01322759
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DOI: https://doi.org/10.1007/BF01322759