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Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2

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Summary

During an outbreak of parvovirus B 19 in 1989 in Northern Ireland, 7580 blood donors were screened for B 19 antigen. Two units screened positive, one of which was obtained for use as viral antigen. A monoclonal antibody (R92F6) made against this antigen was specific for B 19 capsid proteins VP 1 and VP 2. The monoclonal antibody was used in the development of 2 anti-B 19 IgM capture enzyme assays. These used a conventional substrate (O-phenylene diamine) and a chemiluminescent signal reagent. There was excellent concordance between the 2 assays. A total of 403 patients sera were tested and 65 sera were positive in each assay.

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Authors and Affiliations

  1. Regional Virus Laboratory, Royal Victoria Hospital, Belfast, UK

    H. J. O'Neill & P. V. Coyle

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  1. H. J. O'Neill
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  2. P. V. Coyle
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O'Neill, H.J., Coyle, P.V. Two anti-parvovirus B 19 IgM capture assays incorporating a mouse monoclonal antibody specific for B 19 viral capsid proteins VP 1 and VP 2. Archives of Virology 123, 125–134 (1992). https://doi.org/10.1007/BF01317143

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  • DOI: https://doi.org/10.1007/BF01317143

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