Summary
Rhinovirus type 14 RNA-dependent RNA polymerase complexes were isolated from microsomal and soluble fraction of infected KB cells. Maximum activities were measured at 6 and 7 hours post inoculation (p.i.) for microsomal and soluble polymerases, respectively. Both polymerase activities are considerably reduced by 8 to 9 hours, p.i., an interval in which thein vivo rate of synthesis of viral RNA is maximal.In vitro RNA products of RNA polymerases in both fractions consist of ribonuclease-sensitive and ribonuclease-resistant RNA of heterogeneous sizes. Detergent treatment of the microsomal RNA polymerase(s) did not affect the total amount of RNA synthesized, the proportion of ribonuclease-sensitive RNA synthesized nor the size of the RNA products. The data suggest that RV14 RNA polymerase complexes are initially associated with membranes but are then irreversibly released into the soluble phase of the cytoplasm; possible explanations for this phenomena are discussed.
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Griffith, M.M., Gauntt, C.J. Rhinovirus type 14 RNA polymerase complexes. Archives of Virology 47, 11–20 (1975). https://doi.org/10.1007/BF01315588
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DOI: https://doi.org/10.1007/BF01315588