Summary
This report presents data showing that several virus isolates recovered in Argentina, mainly from calves with non-purulent meningo-encephalitis, represent a hitherto unrecognized antigenic variant of BHV-1. The following experimental approaches have been adopted to demonstrate both the unique features among and the relatedness with BHV-1 of these isolates: i) crossed serum neutralization test with rabbit immune sera, ii) analysis by SDS-polyacrylamide gel electrophoresis of radio-labeled virus induced polypeptides and glycoproteins, iii) discriminating reactivity of a panel of monoclonal antibodies which recognize known virus types, and iv) restriction endonuclease analysis of viral DNA. Another strain of BHV-1, which exhibits a specific neuropathogenic potential [Hall et al., Austral. Vet. J.42, 229–237 (1966)] shares all major features with the viral strains originating from Argentina.
Our results imply that antigenic variants of BHV-1 exist and that they can be accurately and easily identified and differentiated by the available methods.
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References
Bagust, T. J.: Comparison of the biological, biophysical and antigenic properties of four strains of infectious bovine rhinotracheitis herpesvirus. J. Comp. Pathol.82, 365–374 (1972).
Bagust, T. J., Clark, L.: Pathogenesis of meningo-encephalitis produced in calves by infectious bovine rhinotracheitis herpesvirus. J. Comp. Pathol.82, 375–383 (1972).
Black, D. N., Slack, G.: A comparison of two strains of infectious bovine rhinotracheitis virus. Arch. ges. Virusforsch.39, 330–337 (1972).
Carrillo, B. J., Ambrogi, A., Schudel, A. A., Vazquez, M., Dahme, E., Pospischil, A.: Meningoencephalitis caused by IBR virus in calves in Argentina. Zbl. Vet. Med.B 30, 327–332 (1983).
Engels, M., Gelderblom, H., Darai, G., Ludwig, H.: Goat herpesviruses: Biological and physicochemical properties. J. gen. Virol.64, 2237–2247 (1983).
French, E. L.: A specific virus encephalitis in calves: isolation and characterization of the causal agent. Austral. Vet. J.38, 216–221 (1962).
French, E. L.: Relationship between infectious bovine rhinotracheitis (IBR) virus and a virus isolated from calves with encephalitis. Austral. Vet. J.38, 555–556 (1962).
Gibbs, E. P. J., Rweyemamu, M. M.: Bovine herpesviruses. Part I. Bovine herpesvirus 1. Vet. Bull.47, 317–343 (1977).
Hall, W. T. K., Simmons, G. C., French, E. L., Snowdon, W. A., Asdell, M.: The pathogenesis of encephalitis caused by the infectious bovine rhinotracheitis virus. Austral. Vet. J.42, 229–237 (1966).
Hill, B. D., Hill, M. W. M., Chung, Y. S., Whittle, R. J.: Meningoencephalitis in calves due to bovine herpesvirus type 1 infection. Austral. Vet. J.61, 242–243 (1984).
Johnston, L. A. Y., Simmons, G. C., McGavin, M. D.: A viral meningoencephalitis in calves. Austral. Vet. J.38, 207–215 (1962).
Lazarowicz, M., Steck, F., Ackermann, M., Kihm, U.: Prüfung von zwei Impfstoffen gegen die Infektiöse Bovine Rhinotracheitis. Schweiz. Arch. Tierheilk.125, 797–808 (1983).
Ludwig, H.: Bovine herpesviruses. In:Roizman, B. (ed.), The Herpesviruses, Vol. 2, 135–314. New York-London: Plenum Press 1983.
Ludwig, H.: Herpesviruses of bovidae: The characterization, grouping and role of different types, including latent viruses. In:Wittmann, G., Gaskell, R. M., Rziha, H.-J. (eds.), Latent Herpes Virus Infections in Veterinary Medicine, 171–189. Boston-The Hague-Dordrecht-Lancaster: Martinus Nijhoff 1984.
Maniatis, T., Fritsch, E. F., Sambrook, J.: Molecular cloning. A laboratory manual. New York: Cold Spring Harbor Laboratory 1982.
McKercher, D. G.: Viruses of other vertebrates. In:Kaplan, A. S. (ed.), The Herpesviruses, 427–499. New York-London: Academic Press 1973.
McKercher, D. G., Bibrack, B., Richards, W. P. C.: Effects of the infectious bovine rhinotracheitis virus on the central nervous system of cattle. J. Am. Vet. Med. Ass.156, 1460–1467 (1970).
Metzler, A. E., Matile, H., Gassmann, U., Engels, M., Wyler, R.: European isolates of bovine herpesvirus 1: a comparison of restriction endonuclease sites, polypeptides, and reactivity with monoclonal antibodies. Arch. Virol.85, 57–69 (1985).
Misra, V., Gilchrist, J. E., Weinmaster, G., Qualtiere, L., van den Hurk, S., Babiuk, L. A.: Herpesvirus-induced “early” glycoprotein: characterization and possible role in immune cytolysis. J. Virol.43, 1046–1054 (1982).
Straub, O. C.: Bovine Herpesvirusinfektionen. Jena: VEB Gustav Fischer 1978.
Watt, J. A., Johnston, W. S., Macleod, N. S., Barlow, R. M.: Infectious bovine rhinotracheitis and encephalitis. Vet. Rec.108, 63–64 (1981).
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Metzler, A.E., Schudel, A.A. & Engels, M. Bovine herpesvirus 1: Molecular and antigenic characteristics of variant viruses isolated from calves with neurological disease. Archives of Virology 87, 205–217 (1986). https://doi.org/10.1007/BF01315300
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DOI: https://doi.org/10.1007/BF01315300