Summary
Antigenicity and immunogenicity of three influenza virus strains A/PR/8/34 (H1N1), A/Hong Kong/1/68 (H3N2) and A/Port Chalmers/1/73 (H3N2) were assayed comparatively with their corresponding neuraminidase isolated by proteolysis, and with the recombinant virus X-42 (Heq1 N2). It was concluded that intranasal immunization of mice with live virus induced heterologous immunity. Except in homologous neuraminidase-vaccinated mice, the subunit always was shown less effective and demonstrated a significantly lower antibody response than the corresponding whole virus.
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Aymard-Henry, M., Coleman, M. T., Dowdle, W. R., Laver, W. G., Schild, G. C., Webster, R. G.: Influenza virus neuraminidase and neuraminidase-inhibition test procedures. Bull. WHO48, 199–202 (1973).
Barry, D. W., Mayner, R. E., Staton, E., Dunlap, R. C., Rastogi, S. C., Hannah, J., Blackburn, R. J., Nortman, D. F., Graze, P. R.: Comparative trial of influenza vaccines: I. Immunogenicity of whole virus and split product vaccines in man. Am. J. Epidemiol.204, 34–46 (1976).
Bottex, C., Chatot, G., Fontanges, R.: Séparation des antigènes de surface du virus A/Hong Kong/1/68 (H3N2) sur gradient de densité à l'aide d'une nouvelle carboxypolypeptidase. Arch. Virol.48, 9–19 (1975).
Bottex, C., Bienvenu, P., Fontanges, R.: Isolement des antigènes de surface de Myxovirus influenza par chromatographie d'affinité. C. R. Acad. Sc. Paris284, 2059–2062 (1977).
Desselberger, U.: Preparation-conditioned changes of the antigenicity of influenza virus neuraminidases. Arch. Virol.53, 335–349 (1977).
Dodge, J. S.: A trial of live intranasal influenza vaccine. N. Z. Med. J.87, 79–82 (1978).
Eckert, E. A.: Alkaline-extracted influenza subunit vaccine. Infect. Immun.14, 1302–1308 (1976).
Ferry, B. J., Evered, M. G., Morrison, E. I.: Different protection rates in various groups of volunteers given subunit influenza virus vaccine in 1976. J. Infect. Dis.139, 237–242 (1979).
Fenton, R. J., Jennings, R., Potter, C. W.: The serological response of experimental animals to inactivated whole and split influenza virus vaccines. J. Biol. Stand.5, 217–231 (1977).
Gross, P. E., Ennis, F. A., Gaerlan, P. F., Denson, L. J., Denning, C. R., Schiffman, D.: A controlled double-bling comparison of reactogenicity, immunogenicity and protective efficacy of whole virus and split-product influenza vaccines in children. J. Infect. Dis.136, 623–632 (1977).
Hammond, M. L., Ferris, A. A., Faine, S., McAvan, T.: Effective protection against Influenza after vaccination with subunit vaccine. Med. J. Aust.1, 301–304 (1978).
Janout, V., Uvizl, M.: Immunological potency of a subunit influenza vaccine in adults. Arch. Virol.53, 171–177 (1977).
Kasel, J. A., Couch, R. B., Six, H. R., Knight, V.: Antigenicity of licensed whole virion and subvirion influenza vaccine in “high risk” persons. Proc. Soc. Exp. Biol. Med.151, 742–747 (1976).
Kasturi, K., Hannoun, C.: Immune responses to influenza virus in rabbits after local immunization I. Local and systemic humoral response. Ann. Microbiol. (Inst. Pasteur)128 A, 97–119 (1977).
Kilbourne, E. D., Schulman, J. L., Couch, R. B., Kasel, J. A.: Monoantigenic immunization and the induction of partial immunity. A new approach to influenza immunization. In:Melnik, J. L. (ed.), International Virology. (2. Proceedings of the 2nd International Congress for Virology), 118–119. Basel: S. Karger 1972.
Laver, W. G., Webster, R. G.: Preparation and immunogenicity of an influenza virus hemagglutinin and neuraminidase subunit vaccine. Virology69, 511–522 (1976).
Lowry, O. H., Rosebrough, N. J., Farr, A. L., Randall, R. J.: Protein measurement with the Folin phenol reagent. J. Biol. Chem.183, 265–275 (1951).
MacLaren, C., Williams, M. S., Bozeman, F. M., Mayner, R. E., Gaubbs, G. E., Barthlow, W. E., Stanton, E., Ennis, F. A.: Comparative antigenicity and immunogenicity of 1976 influenza virus vaccines: results of mouse protection experiments. J. Biol. Stand.6, 315–331 (1978).
Masurel, N., Baars, H. J., Frankena, H.: Vaccination and protection experiments in mice with the human A. 1957 and A. 1968 strains and the equi-2 strain of influenza vaccine. Antonie van Leeuwenhoek.39, 461–471 (1973).
Murphy, B. R., Kasel, J. A., Chanock, R. M.: Association of serum anti-neuraminidase antibody with resistance to influenza in man. New Engl. J. Med.286, 1325–1332 (1972).
Ortbals, D. W., Liebhaber, H.: Comparison of immunogenicity of a whole virion and a subunit influenza vaccine in adults. J. Clin. Microbiol.8, 431–438 (1978).
Palese, P., Schulman, J. L., Tobita, K.: The requirement of neuraminidase activity for influenza virus replication. Behring Inst. Mitt.55, 11–18 (1974).
Palmer, D. F., Dowdle, W. R., Coleman, M. T., Schild, G. C.: Advanced laboratory technicals for immunological diagnostic. U.S. Dept. Hlth. Welfare. PHS. Atlanta. Immunology Ser. no. 6. Procedural guide, 25–62 (1975).
Schild, G. C.: Influenza virus antigenic composition and immune response. Post. Med. J.55, 87–98 (1979).
Warren, L.: The thiobarbituric acid assay of sialic acid. J. Biol. Chem.234, 1971–1975 (1959).
Yamane, N., Odagiri, T., Arikawa, J., Kumasaka, M., Ishida, N.: Effect of specific immunity to viral neuraminidase on subsequent influenza virus infection in man. Microbiol. Immunol.23, 565–567 (1979).
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Bottex, C., Burckhart, M.F. & Fontanges, R. Comparative immunogenicity of live influenza viruses and their solubilized neuraminidases: Results of mouse protection experiments. Archives of Virology 70, 83–89 (1981). https://doi.org/10.1007/BF01315002
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DOI: https://doi.org/10.1007/BF01315002