Summary
The Bryan High Titer strain of Rous Sarcoma Virus (BH-RSV) is a deletion mutant in the env-gene coding for the viral envelope glycoproteins gp35 and gp85. In this report experimental evidence is described that cells, transformed by BH-RSV, express a glycoprotein immunologically related to gp85.
Animals bearing BH-RSV induced tumors produce antibodies reacting with gp85 of nondefective RSV. Lysates of a BH-RSV transformed quail cell line, R(−): Q, inhibit the immunoprecipitation of gp85 by antibodies against the group-specific determinant of gp85. In R(−): Q cell lysates and in the culture supernatant a glycoprotein of an apparent molecular weight 40,000 (gp40) is found that reacts with monospecific antisera against gp85 of nondefective RSV. In newly synthesized BH-RSV a gp40 associated with the virion is detectable but is easily lost during purification of the virus. Further, a 95k glycoprotein and a 95k phosphoprotein are specifically precipitated from R(−): Q cells by an antiserum against gp85.
From these results we conclude that the deletion of the env-gene is incomplete such that part of gp85, bearing group-specific antigenic determinants, is expressed in BH-RSV transformed cells.
Analysis of BH-RSV particles freshly harvested from R(−): Q cells reveals that they contain almost exclusively the gag-precursor pr76 and little or no processed gag-proteins. Therefore the R(−): Q cell line seems to be suitable for the study of virus maturation occuring after the budding process.
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Pauli, G., Fleischer, B. Evidence for a Gp85-related glycoprotein in cells transformed by the Bryan High Titer strain of Rous Sarcoma Virus. Archives of Virology 68, 173–187 (1981). https://doi.org/10.1007/BF01314570
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DOI: https://doi.org/10.1007/BF01314570