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Prostaglandin requirements are greater for protection in cold restraint-induced than alcohol-induced gastric mucosal injury

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Abstract

Exogenous prostaglandins inhibit visible gastric muscosal lesions produced by both absolute ethanol and cold restraint in the rat. Pretreatment with “mild irritants” significantly reduces the magnitude of ethanol-induced lesions presumably by stimulating endogenous prostanoid production. The effect of mild irritant pretreatment on cold restraint-induced lesion formation has not been previously reported. This study was designed to compare the protective effect of pretreatment with two “mild irritants,” 4% NaCl and 0.35 M HCl, and the synthetic prostanoid, 16,16 dimethyl PGE2)16,16-dm PGE2), on lesions produced by cold restraint or absolute ethanol. Pretreatment with both mild irritants produced complete visible protection against ethanol-induced injury but had variable effects against cold restraint-induced injury. Whereas 5 μg/kg 16,16-dmPGE2 provided complete visible protection against ethanol-induced injury, 20 μg/kg 16,16-dmPGE2 was required for complete visible protection against cold restraint-induced injury. We conclude that prostaglandin requirements for protection against cold restraint injury are greater than for protection against ethanol-induced gastric mucosal injury.

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References

  1. Robert A, Nezamis JE, Lancaster C, Hanchar A: Cytoprotection by prostaglandins in rats: Prevention of gastric necrosis produced by alcohol, HCl, NaOH, hypertonic NaCl, and thermal injury. Gastroenterology 77:433–443, 1979

    Google Scholar 

  2. Senay EC, Levine RJ: Synergism between cold and restraint for rapid production of stress ulcers in rats. Proc Soc Exp Biol Med 124:1221–1223, 1967

    Google Scholar 

  3. Lacy ER, Ito S: Microscopic analysis of ethanol damage to rat gastric mucosa after treatment with a prostaglandin. Gastroenterology 83:619–625, 1982

    Google Scholar 

  4. Goldman H, Rosohoff CB: Pathogenesis of acute gastric stress ulcers. Am J Pathol 52:227–236, 1968

    Google Scholar 

  5. Mersereau WA, Hinchey J: Hypothermia-induced gastric hypercontractility in the genesis of the restraint ulcer. Can J Surg 24:622–625, 1981

    Google Scholar 

  6. Karawada Y, Lambek J, Matsumoto T: Pathophysiology of stress ulcer and its prevention. II Prostaglandin E and microcirculatory responses in stress ulcer. Am J Surg 129:217–222, 1975

    Google Scholar 

  7. Robert A, Lancaster C, Hanchar AJ, Nezamis JE: Mild irritants prevent gastric necrosis through prostaglandin formation: A histological study. Gastroenterology 74:1086, 1978 (abstract)

    Google Scholar 

  8. Robert A, Nezamis JE, Lancaster C, Davis JP, Field SO, Hanchar J: Mild irritants prevent gastric necrosis through “adaptive cytoprotection” mediated by prostaglandins. Am J Physiol 245:G113-G121, 1983

    Google Scholar 

  9. Chaudhury TK, Robert A: Prevention by mild irritants of gastric necrosis produced in rats by sodium taurocholate. Dig Dis Sci 25:830–836, 1980

    Google Scholar 

  10. Hanson HM, Brodie DA: Use of the restrained rat technique for study of the anti-ulcer effect of drugs. J Appl Physiol 15:291–294, 1960

    Google Scholar 

  11. Watanabe K: Some pharmacological factors involved in formation and prevention of stress ulcer in rats. Chem Pharmacol Bull 14:101–107, 1966

    Google Scholar 

  12. Goto Y, Watanabe K: Inhibitory mechanism of calcitonin on vagally induced gastric acid secretion in the rat in relation to its antiulcer activity.In Advances in Experimental Ulcer. S Umehara, H Ito (eds). Tokyo, ICEU Press, 1980, pp 337–347

    Google Scholar 

  13. Menguy R: Effect of restraint stress on gastric secretion in the rat. Am J Dig Dis 11:911–916, 1960

    Google Scholar 

  14. Dai S, Ogle CW: Gastric ulcers induced by acid accumulation and by stress in pylorus-occluded rats. Eur J Pharmacol 26:15–21, 1974

    Google Scholar 

  15. Levine BA, Sirinek KR, Pruitt BA: Cimetidine protects against stress-induced gastric injury augmented by mucosal barrier breakers. Am J Surg 137(3):328–331, 1979

    Google Scholar 

  16. Davenport HW: Physiology of the Digestive Tract, Year Book Medical Publ., Chicago, 1977, p 124

    Google Scholar 

  17. Robert A: Prostaglandins and the gastrointestinal tract.In Physiology of the Gastrointestinal Tract. LR Johnson (ed). New York, Raven Press, 1981, pp 1407–1434

    Google Scholar 

  18. Watanabe K, Goto Y, Nobuyuki H: Continous measurement of gastric bleeding in the rat subjected to restraint and water-immersion stress.In Advances in Experimental Ulcer. S Umehara, H Ito (eds). Japan, ICEU, 1981, pp 82–88

    Google Scholar 

  19. Robert A, Schultz JR, Nezamis JE, Lancaster E: Gastric antisecretory and antiulcer properties of PGE2, 15-methyl PGE2 and 16,16-dimethyl PGE2. Gastroenterology 70:359–370, 1976

    Google Scholar 

  20. Yano S, Akahane M, Harada N: Role of gastric motility in the development of stress induced gastric lesions of rats. Jpn J Pharmacol 28:607–615, 1978

    Google Scholar 

  21. Chawla RC, Eisenberg MM: Prostaglandin inhibition of denervated antral motility in dogs. Proc Sci Exp Biol Med 132:1081–1086, 1969

    Google Scholar 

  22. Sanders KM: Endogenous prostaglandin E and contractile activity of isolated ileal smooth muscle. Am J Physiol 3(2):E209-E212, 1978

    Google Scholar 

  23. Sanders KM, Szurszewski JH: Does endogenous prostaglandin affect gastric antral motility. Am J Physiol 241:G191-G195, 1981

    Google Scholar 

  24. Svanes K, Critchlow J, Takeuchi K, Magee S, Ito S, Silen W: Factors influencing reconstitution of frog gastric mucosa: role of prostaglandins.In Mechanisms of Mucosal Protection in the Upper Gastrointestinal Tract. A Allan, Flemstrom G, Garner A, Silen W, Turnberg LA (eds). New York, Raven Press, 1984, pp 33–40

    Google Scholar 

  25. Basso N, Materia A, Forlini A, Jaffe BM: Prostaglandin generation in the gastric mucosa of rats with stress ulcer. Surgery 94:104–108, 1983

    Google Scholar 

  26. Konturek S, Brzozowski T, Piastucki I, Radecki T, Dembinski A, Dembinska-kiec A: Role of locally generated prostaglandins in adaptive gastric cytoprotection. Dig Dis Sci 27:967–971, 1982

    Google Scholar 

  27. Robert A, Lancaster JE, Nezamis JE, Davis JP, Field SO: Mild irritants stimulate prostaglandin synthesis by the gastric mucosa: Mechanism of adaptive cytoprotection. Gastroenterology 84:A28, 1983 (abstract)

    Google Scholar 

  28. Okabe S, Takeuchi K, Kunimi H, Kanno M, Kawashima M: Effects of an antiulcer drug, sucralfate (a basic aluminum salt of sulfated disaccharide), on experimental gastric lesions and gastric secretion in rats. Dig Dis Sci 28:1034–1042, 1983

    Google Scholar 

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Authors and Affiliations

  1. Psychiatric, Surgical and Research Services, and Center for Ulcer Research and Education, West Los Angeles, Wadsworth Division, Veterans Administration Medical Center, Wilshire and Sawtelle Blvds., 90073, Los Angeles, California

    Thomas Garrick MD, Elizabeth Kolve BS & Gordon L. Kauffman Jr MD

  2. UCLA School of Medicine, Wilshire and Sawtelle Blvds., 90073, Los Angeles, California

    Thomas Garrick MD, Elizabeth Kolve BS & Gordon L. Kauffman Jr MD

Authors
  1. Thomas Garrick MD
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  2. Elizabeth Kolve BS
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  3. Gordon L. Kauffman Jr MD
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Additional information

This work was supported by National Institutes of Health grants BRSG 4-434400-32602, NIADDK AM 27465 and AM 17328.

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Garrick, T., Kolve, E. & Kauffman, G.L. Prostaglandin requirements are greater for protection in cold restraint-induced than alcohol-induced gastric mucosal injury. Digest Dis Sci 31, 401–405 (1986). https://doi.org/10.1007/BF01311676

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  • DOI: https://doi.org/10.1007/BF01311676

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