Summary
In an attempt to produce Hog Cholera virus (HCV) preparations of high titre, optimal growth and trypsinization conditions of PK-15 microcarrier cell cultures were defined. Infecting a PK-15 Cytodex 3® microcarrier culture with HCV increased the yield of virus more than 10 times compared with conventional monolayer culture in Roux flasks.
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Caij, A., De Smet, A., Dubois, N. et al. High titre Hog Cholera virus production of Cytodex 3® microcarrier cultures. Archives of Virology 105, 113–118 (1989). https://doi.org/10.1007/BF01311121
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DOI: https://doi.org/10.1007/BF01311121