Summary
The genes coding for the rotavirus major neutralizing protein, VP7, from 5 Australian porcine rotaviruses representing glycoprotein (i.e. VP7 or G) serotypes 3, 4, and 5, were sequenced. The genes were each 1,062 nucleotides long with two long open reading frames for proteins of either 326 or 297 amino acids and containing only one potential glycosylation site at amino acid position 69. When compared to the corresponding genes of human viruses, the porcine genes showed very high nucleotide and deduced amino acid homology. Sequence comparison also revealed that Australian porcine rotaviruses of G serotype 4 and 5 were similar to the corresponding porcine strains found in the U.S.A. and U.K., while G serotype 3 and 4 porcine rotaviruses were closely related to human G serotype 3 strain, RV-3 and serotype 4 strain, ST-3, respectively. These Australian rotavirus VP7 sequences were found to correlate with serological data we reported previously.
Similar content being viewed by others
References
Albert MJ, Bishop RF (1984) Cultivation of human rotaviruses in cell culture. J Med Virol 13: 377–383
Albert MJ, Unicomb LE, Tzipori SR, Bishop RF (1987) Isolation and serotyping of animal rotaviruses and antigenic comparison with human rotaviruses. Arch Virol 93: 123–130
Alexander S, Elder JH (1984) Carbohydrate dramatically influences immune reactivity of antisera to viral glycoprotein antigens. Science 226: 1328–1330
Bohl EH, Theil KW, Saif L (1984) Isolation and serotyping of porcine rotaviruses and antigenic comparison with other rotaviruses. J Clin Microbiol 19: 105–111
Both GW, Mattick JS, Bellamy AR (1983) Serotype-specific glycoprotein of Simian-11 rotavirus: coding assignment and gene sequence. Proc Natl Acad Sci USA 80: 3091–3095
Caust J, Dyall-Smith ML, Lazdins I, Holmes IH (1987) Glycosylation, an important modifier of rotavirus antigenicity. Arch Virol 96: 123–124
Cohen J, Laporte J, Charpilienne J, Scherrer R (1979) Activation of rotavirus RNA polymerase by calcium chelation. Arch Virol 60: 177–186
DeBorde DC, Naeve CW, Herlocher ML, Maassab HF (1986) Resolution of a common RNA sequence ambiguity by terminal deoxynucleotidyl transferase. Anal Biochem 157: 275–282
Dyall-Smith ML, Holmes IH (1981) Gene coding assignment of rotavirus double-stranded RNA segments 10 and 11. J Virol 38: 1099–1103
Dyall-Smith ML, Lazdins I, Tregear GW, Holmes IH (1986) Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci USA 83: 3465–3468
Esparza J, Gil F (1978) A study on the ultrastructure of human rotavirus. Virology 91: 141–159
Estes MK, Palmer EL, Obijeski JF (1983) Rotaviruses: a review. Curr Top Microbiol Immunol 105: 123–184
Flewett TH, Woode GN (1978) The Rotaviruses. Arch Virol 57: 1–23
Glass RI, Keith J, Nakagomi O, Nakagomi T, Askaa J, Kapikian AZ, Chanock RM, Flores J (1985) Nucleotide sequence of the structural glycoprotein VP7 gene of Nebraska calf diarrhoea virus (rotavirus): comparison with homologous genes from four strains of human and animal rotaviruses. Virology 41: 292–298
Gorziglia M, Aguirre Y, Hoshino Y, Esparza J, Blumentals I, Askaa, J, Thompson M, Glass RI, Kapikian AZ, Chanock RM (1986) VP7 serotype-specific glycoprotein of OSU porcine rotavirus: coding assignment and gene sequence. J Gen Virol 67: 2445–2454
Gorziglia M, Nishikawa K, Green KY, Taniguchi K (1988) Gene sequence of the VP7 serotype specific glycoprotein of Gottfried porcine rotavirus. Nucleic Acids Res 16: 775
Green KY, Midthun K, Gorziglia M, Hoshino Y, Kapikian AZ, Chanock RM, Flores J (1987) Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology 161: 153–157
Greenberg HB, Macauliffe HV, Valdesuso J, Wyatt RG, Flores J, Kalica A, Hoshino Y, Singh N (1983) Serological analysis of the subgroup proteins of rotavirus using monoclonal antibodies. Infect Immun 39: 91–99
Holmes IH (1983) Rotaviruses. In: Joklik WK (ed) The Reoviridae. Plenum, New York, pp 359–423
Hoshino Y, Wyatt RG, Greenberg HB, Flores J, Kapikian AZ (1984) Serotypic similarity of rotavirus of mammalian and avian origin as studied by plaque-reduction neutralization. J Infect Dis 149: 694–702
Hoshino Y, Sereno M, Midthun K, Flores J, Kapikian AZ, Chanock RM (1985) Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci USA 82: 8701–8704
Kantharidis P, Dyall-Smith ML, Holmes IH (1987) Marked sequence variation between segment 4 genes of human RV5 and simian SA 11 rotaviruses. Arch Virol 93: 111–121
Kalica AR, Sereno MM, Wyatt RG, Mebus CA, Chanock RM, Kapikian AZ (1978) Comparison of human and animal rotavirus strains by gel electrophoresis of viral RNA. Virology 91: 141–150
Kalica AR, Flores J, Greenberg HB (1983) Identification of the rotaviral gene that codes for hemagglutination and protease-enhanced plaque formation. Virology 125: 194
Kapikian AZ, Chanock RM (1985) Rotaviruses. In: Fields BN (ed) Virology. Raven Press, New York, pp 863–906
Kozak M (1984) Point mutations close to the AUG initiation codon affect the efficiency of translation of rat preproinsulin in vivo. Nature 308: 241–246
Mackow ER, Shaw RD, Matsui SM, Vo PT, Benfield D, Greenberg HB (1988) Characterization of homotypic and heterotypic VP7 neutralization sites of Rhesus rotavirus. Virology 165: 511–517
Malherbe HH, Strickland-Cholmley M (1967) Simian virus SA11 and the related ‘O’ agent. Arch Ges Virusforsch 22: 235–245
Nagesha HS, Holmes IH (1988) New porcine rotavirus serotype antigenically related to human rotavirus serotype 3. J Clin Microbiol 26: 171–174
Nagesha HS, Brown LE, Holmes IH (1989) Neutralizing monoclonal antibodies against three serotypes of porcine rotaviruses. J Virol 63: 3545–3549
Richardson MA, Iwamoto A, Ikegami N, Nomoto A, Furuichi Y (1984) Nucleotide sequence of the gene encoding the serotype-specific antigen of human (Wa) rotavirus. Comparison with the homologous genes from simian SA11 and UK bovine rotaviruses. J Virol 51: 860–862
Rodger SM, Craven JA, Williams I (1975) Demonstration of reovirus-like particles in intestinal contents of piglets with diarrhoea. Aust Vet J 51: 536
Rodger SM, Holmes IH (1979) Comparison of the genomes of simian, bovine, and human rotaviruses by gel electrophoresis and detection of genomic variation among bovine isolates. J Virol 30: 839–849
Roseto A, Escaig J, Delain E, Cohen J, Scherrer R (1979) Structure of rotaviruses as studied by freeze-drying technique. Virology 98: 471–475
Ruiz AM, Lopez IV, Lopez S, Espejo RT, Arias CF (1988) Molecular and antigenic characterization of porcine rotavirus YM, a possible new rotavirus serotype. J Clin Microbiol 62: 4331–4336
Smith ML, Lazdins I, Holmes IH (1980) Coding assignments of double-stranded RNA segments of SA11 rotavirus established by in vitro translation. J Virol 33: 976–982
Taniguchi K, Morita Y, Urasawa T, Urasawa S (1987) Cross reactive neutralization epitopes on VP3 of human rotaviruses: analysis with monoclonal antibodies and antigenic variants. J Virol 61: 1726–1730
Taniguchi K, Hoshino Y, Nishikawa K, Green KY, Maloy WL, Urasawa S, Kapikian AZ, Chanock RM, Gorziglia M (1988) Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol 62: 1870–1874
Wyatt RG, James HD, Pittman AL, Hoshino Y, Greenberg HB, Kalica AR, Flores J, Kapikian AZ (1983) Direct isolation in cell culture of human rotaviruses and their characterization into four serotypes. J Clin Microbiol 18: 310–317
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Huang, J., Nagesha, H.S., Dyall-Smith, M.L. et al. Comparative sequence analysis of VP7 genes from five Australian porcine rotaviruses. Archives of Virology 109, 173–183 (1989). https://doi.org/10.1007/BF01311079
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF01311079