Summary
Consecutive serum samples collected from 235 patients with Haemorrhagic Fever with Renal Syndrome (HFRS), between two days and two years after onset of disease, have been analysed for the presence of IgG and IgM type of antibodies specific for Hanta-viruses. The sera were screened in parallel by a newly developed indirect Immuno Enzyme Assay (EIA) in parallel with Indirect Immunofluorescent Antibody Assay (IFA). In both tests the Hantaan virus strain 76–118 was used as the antigen. The EIA was much more sensitive than the IFA test for the detection of IgM type antibodies. With the indirect EIA IgM type antibodies against Hantaan virus 76–118 have been detected in HFRS patient's sera from the second day of illness indicating the usefulness of this test for the early serological diagnosis of this disease.
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References
Arikawa J (1984) Sero-epidemiological study of HFRS inRattus norvegicus by IFA, CF, and ELISA. Arthropod-borne virus information exchange, March 12, 1984
Bashkirtsev VN, Tkachenko EA, Dzagurova TK, Ryltseva EV (1984) Isolation of strains of haemorrhagic fever with renal syndrome virus in cell culture (in Russian). Vopr Virusol 4: 497–502
Dournon E, Moriniere B, Matherron S, Girard PM, Gonzalez J-P, Hirsch F, McCormick JB (1984) HFRS after a wild rodent bite in the Hautesavoie—and risk of exposure to Hantaan-like virus in a Paris laboratory. Lancet 8378: 676–677
Goldgaber D, Lee PW, Gibbs CJ, Gajdusek DC (1983) Quantitation of Hantaan virus infectivity, antigen and antibody by immunoenzym methods. First International Symposium on Public Health in Asia and the Pacific Basin, Honolulu, Hawaii, March 7–11, 1983, Abstract 94
Hurault De Ligny B, Prieur JP, Schmitt JL, Kessler M, Canton Ph, Dureux JB, Rollin PE, Sureau P, McCormick JB, Dournon E (1984) The new cases of HFRS in northeastern France. Lancet 8407: 864–865
Ivanov AP, Rezapkin GV, Dzagurova TK, Tkachenko EA (1984) Indirect solid-phase immunosorbent assay for detection of arenavirus antigens and antibodies. Acta Virol 28: 240–245
Knez V, Stewart JA, Ziegler DV (1976) Cytomegalovirus specific IgM and IgG response in humans studied by radioimmunoassay. J Immunol 117: 206–213
Lee HW (1982) Korean haemorrhagic fever. Prog Med Virol 28: 96–113
Nakane PK, Kawaoi A (1974) Peroxidase-labelled antibody: a new method of conjugation. J Histochem Cytochem 22: 1084–1091
Song Gan, Hang Chang-Shou, Liao Hua-Xin, Fu Jian-Lin (1984) Antigenic comparison of virus strains of mild and classical types of epidemic haemorrhagic fever isolated in China and adaptation of these to cultures of normal cells. Lancet 8378: 677–678
Sugiyama K, Matsuura Y, Morita C, Shiga S, Akao Y, Komatsu T, Kitamura T (1984) An immune adherence assay for discrimination between etiologic agents of HFRS. J Infect Dis 149 1: 67–73
Tkachenko EA, Ivanov AP, Dzagurova TK, Donets MA, Rezapkin GV, Lescchinskaya EV (1981) Immunosorbent assays for diagnosis of haemorrhagic fever with renal syndrome. Lancet 8240: 257–258
Tsai TF, Yi Wei Tang, Shan Lian Hu, Ke Long Ye, Guo Liang Chen, Zhi Yi Xu (1984) Hemagglutination-inhibition antibody in hemorrhagic fever with renal syndrome. J Infect Dis 150 6: 895–898
van der Groen G (1985) Haemorrhagic fever with renal syndrome recent developments. Ann Soc Belge Med Trop 65: 121–135
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Ivanov, A.P., Tkachenko, E.A., Petrov, V.A. et al. Enzyme immuno assay for the detection of virus specific IgG and IgM antibody in patients with Haemorrhagic Fever with Renal Syndrome. Archives of Virology 100, 1–7 (1988). https://doi.org/10.1007/BF01310902
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DOI: https://doi.org/10.1007/BF01310902