Skip to main content
SpringerLink
Log in

Detection of human cytomegalovirus genome in uterus tissue

  • Original Papers
  • Published:
Archives of Virology Aims and scope Submit manuscript

Summary

To analyze persistent infection by human cytomegalovirus (HCMV) in vivo, specimens obtained from various sources and autopsied organs were examined for the presence of HCMV DNA, mRNA transcripts and antigens by polymerase chain reaction, in situ hybridization and immunostaining. The HCMV genome was detected in lung, liver, kidney, and blood vessels at an average positive rate of 15%. The highest PCR-positive rate was observed with cervical smears. Subsequent examination of uterus tissues from patients with myoma revealed HCMV transcripts and antigens in glandular epithelial cells, leukocytes, endothelial cells, and others, indicating productive HCMV infection of cervical tissue.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Ahmed R, Stevens JG (1991) Viral persistence. In: Fields BN, Knipe DM (eds) Fundamental virology, 2nd edn. Raven Press, New York, pp 241–265

    Google Scholar 

  2. Balfour Jr HH, Slade MS, Kalis JM, Howard RJ, Simmons RL, Najarian JS (1977) Viral infections in renal transplant donors and their recipients: A prospective study. Surgery 81: 487–492

    Google Scholar 

  3. Benyesh-Melnick M, Dessy SI, Fernbach DJ (1964) Cytomegalovirus in children with acute leukemia and in other children. Proc Soc Exp Biol Med 117: 624–630

    Google Scholar 

  4. Betts RF, Freeman RB, Douglas Jr RG, Talley JE, Rundell B (1975) Transmission of cytomegalovirus infection with renal allograft. Kidney Int 8: 387–394

    Google Scholar 

  5. Dankner WM, MuCutchan JA, Richman DD, Hirata K, Spector SA (1990) Localization of human cytomegalovirus in peripheral blood leukocytes by in situ hybridization. J Infect Dis 161: 31–36

    Google Scholar 

  6. Demmler GJ, Buffone GJ, Schimbor CM, May RA (1988) Detection of cytomegalovirus in urine from newborns by using polymerase chain reaction DNA amplification. J Infect Dis 158: 1177–1184

    Google Scholar 

  7. Dummer JS, White LF, Ho M (1985) The morbidity of cytomegalovirus infection in heart and heart-lung transplant recipients on cyclo-sporine. J Infect Dis 152: 1182–1191

    Google Scholar 

  8. Gnann Jr JW, Ahlmen J, Svalander C, Olding L, Oldstone MBA, Nelson NA (1988) Inflammatory cell in transplanted kidneys are infected by human cytomegalovirus. Am J Pathol 132: 239–248

    Google Scholar 

  9. Goodpasture EW, Talbot FB (1921) Concerning the nature of “protozoan-like” cells in certain lesions of infancy. Am J Dis Child 21: 415–425

    Google Scholar 

  10. Grazia-Revello M, Percivalle E, DiMatteo A, Morini F, Gerna G (1992) Nuclear expression of the lower matrix protein of human cytomegalovirus in peripheral blood leukocytes of immunocompromised viraemic patients. J Gen Virol 73: 437–442

    Google Scholar 

  11. Ho M (1990) Epidemiology of cytomegalovirus infections. Rev Infect Dis 12: 701–710

    Google Scholar 

  12. Ho M (1991) Epidemiology of CMV in man. In: Cytomegalovirus: biology and infection, 2nd edn. Plenum Press, New York, pp 155–187

    Google Scholar 

  13. Ho M, Suwansirikal S, Dowling JN, Youngblood LA, Armstrong JA (1975) The transplanted kidney is a source of cytomegalovirus infection. N Engl J Med 293: 1109–1112

    Google Scholar 

  14. Jablonski E, Moomaw EW, Tullis RH, Ruth JL (1986) Preparation of oligonucleotidealkaline phosphatase conjugates and their use as hybridization probes. Nucleic Acids Res 14: 6113–6126

    Google Scholar 

  15. Knox CE, Pass RF, Reynolds DW, Stagno S, Alford CA (1979) Comparative prevalence of subclinical cytomegalovirus and herpes simplex virus infections in the genital and urinary tracts of low-income, urban women. J Infect Dis 140: 419–422

    Google Scholar 

  16. Krech U (1973) Complement-fixing antibodies against cytomegalovirus in different parts of the world. Bull World Health Organ 49: 103–106

    Google Scholar 

  17. LaFemina RL, Hayward GS (1986) Constitutive and retinoic acid inducible expression of cytomegalovirus immediate-early genes in human teratocarcinoma cells. J Virol 58: 434–440

    Google Scholar 

  18. Maniatis T, Fritsch EF, Sambrook J (1982) Molecular cloning: a laboratory manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, pp 85–93

    Google Scholar 

  19. Mocarski ES, Stinski MF (1979) Persistence of the cytomegalovirus genome in human cells. J Virol 31: 761–775

    Google Scholar 

  20. Montgomery RL, Youngblood LA, Medaris Jr DN (1972) Recovery of cytomegalovirus from the cervix in pregnancy. Pediatrics 49: 524–531

    Google Scholar 

  21. Murayama T, Furukawa T, Jisaki F, Tateno E, Hirai K (1990) Application for rapid detection of human cytomegalovirus infection by polymerase chain reaction DNA amplification. Clin Virol 18: 345–348 (in Japanese)

    Google Scholar 

  22. Murayama T, Jisaki F, Ayata M, Sakamuro D, Hironaka T, Hirai K, Tsuchiya N, Ito K, Furukawa T (1992) Cytomegalovirus genomes demonstrated by polymerase chain reaction in synovial fluid from rheumatoid arthritis patients. Clin Exp Rheumatol 10: 161–164

    Google Scholar 

  23. Numazaki Y, Yano N, Morizuka T, Takai S, Ishida N (1970) Primary infection with human cytomegalovirus: virus isolation from healthy infants and pregnant women. Am J Epidemiol 91: 410–417

    Google Scholar 

  24. Paya CV, Hermans PE, Wiesner RH, Ludwig J, Smith TF, Rakela J, Krom RAF (1989) Cytomegalovirus hepatitis in liver transplantation: prospective analysis of 93 consecutive orthotopic liver transplantation. J Infect Dis 160: 752–758

    Google Scholar 

  25. Reusser P, Fisher LD, Buckner DC, Thomas DE, Meyers JD (1990) Cytomegalovirus infection after autologous bone marrow transplantation: occurrence of cytomegalovirus disease and effect on engraftment. Blood 75: 1888–1894

    Google Scholar 

  26. Rice GPA, Schrier RD, Oldstone MBA (1984) Cytomegalovirus infects human lymphocytes and monocytes: virus expression is restricted to immediate-early gene products. Proc Natl Acad Sci USA 81: 6134–6138

    Google Scholar 

  27. Ruger B, Klages S, Walla B, Albrecht J, Fleckenstein B, Tomlinson P, Barrell B (1987) Primary structure and transcription of the genes coding for the two phosphoproteins pp 65 and pp 71 of human cytomegalovirus. J Virol 61: 446–453

    Google Scholar 

  28. Schrier RD, Nelson JA, Oldstone MBA (1985) Detection of human cytomegalovirus in peripheral blood lymphocytes in a natural infection. Science 230: 1048–1051

    Google Scholar 

  29. Shen CY, Ho MS, Chang SF, Yen MS, Ng HT, Huang ES, Wu CW (1993) High rate of concurrent genital infections with human cytomegalovirus and human papilloma viruses in cervical cancer patients. J Infect Dis 163: 449–452

    Google Scholar 

  30. Sixbey JW, Nedrud JG, Raab-Traub N, Hanes RA, Pagano JS (1984) Epstein-Barr virus replication in oropharyngeal epithelial cells. N Engl J Med 310: 1225–1230

    Google Scholar 

  31. Stagno S, Reynolds D, Tsiantos A, Fucillo DA, Smith R, Tiller M, Alford CA (1975) Cervical cytomegalovirus excretion in pregnant and nonpregnant women: suppression in early gestation. J Infect Dis 131: 522–527

    Google Scholar 

  32. Stanier P, Taylor DL, Kitchen AD, Wales N, Tryhorn Y, Tyms AS (1989) Persistence of cytomegalovirus in mononuclear cells in peripheral blood for blood donors. Br Med J 299: 897–898

    Google Scholar 

  33. Stenberg RM, Thomsen DR, Stinski MF (1984) Structural analysis of the major imemdiate early gene of human cytomegalovirus. J Virol 49: 190–199

    Google Scholar 

  34. Taylor-Wiedeman J, Sissons JGP, Borysiewicz LK, Sinclair JH (1991) Monocytes are a major site of persistence of human cytomegalovirus in peripheral blood mononuclear cells. J Gen Virol 72: 2059–2064

    Google Scholar 

  35. Weinshenkev BG, Wilton S, Rice GPA (1988) Phorbol ester-induced differentiation permits productive human cytomegalovirus infection in a monocytic cell line. J Immunol 140: 1625–1631

    Google Scholar 

  36. Weller TH, Macauley JC, Craig JM, Wirth P (1957) Isolation of intranuclear inclusion producing agents from infants with illnesses resembling cytomegalic inclusion disease. Proc Soc Exp Biol Med 94: 4–12

    Google Scholar 

  37. Wu T-C, Lee WA, Pizzorno MC, Au W-C, Chen Y-J, Hruban RH, Hutchins GM, Hayward GS (1992) Localization of the human cytomegalovirus 2.7-kb major early β-gene transcripts by RNA in situ hybridization in permissive and nonpermissive infections. Am J Pathol 141: 1247–1254

    Google Scholar 

  38. Yamaguchi Y, Hironaka T, Kajiwara M, Tateno E, Kita H, Hirai K (1992) Increased sensitivity for detection of human cytomegalovirus in urine by removal of inhibitors for the polymerase chain reaction. J Virol Methods 37: 209–218

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Microbiology, Kanazawa Medical University, Uchinada, Ishikawa, Japan

    T. Furukawa, D. Sakamuro, T. Takegami & T. Murayama

  2. Department of Pathology, Kanazawa Medical University, Uchinada, Ishikawa, Japan

    F. Jisaki

Authors
  1. T. Furukawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. F. Jisaki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. D. Sakamuro
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. T. Takegami
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. T. Murayama
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

Presented in part: at the 4th International Cytomegalovirus Conference, Paris, France, April 1993.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Furukawa, T., Jisaki, F., Sakamuro, D. et al. Detection of human cytomegalovirus genome in uterus tissue. Archives of Virology 135, 265–277 (1994). https://doi.org/10.1007/BF01310013

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01310013

Keywords

Search

Navigation