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Specific changes in the M1 protein during adaptation of influenza virus to mouse

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Summary

The matrix (M) gene of influenza virus has been implicated as a determinant of virulence for mouse brain and lung. Comparison of the M gene sequences of the mouse brain adapted variants A/NWS/33 and A/WSN/33 to their parent, A/WS/33, identified two specific amino acid substitutions in the M1 protein which correlated with virulence for mouse: Ala41 → Val and Thr139 → Ala.

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References

  1. Baez M, Palese P, Kilbourne ED (1980) Gene composition of high-yielding influenza vaccine strains obtained by recombination. J Infect Dis 141: 362–369

    Google Scholar 

  2. Baylor NW, Li Y, Ye Z, Wagner RR (1988) Transient expression and sequence of the matrix (M1) gene of WSN influenza A virus in a vaccinia vector. Virology 163: 618–621

    Google Scholar 

  3. Bean WJ, Kawaoka Y, Wood JM, Pearson JE, Webster RG (1985) Characterisation of virulent and avirulent A/Chicken/Pennsylvania/83 influenza A viruses: potential role of defective interfering RNAs in nature. J Virol 54: 151–160

    Google Scholar 

  4. Brown EG (1990) Increased virulence of a mouse-adapted variant of influenza A/FM/1/47 virus is controlled by mutations in genome segments 4, 5, 7, and 8. J Virol 64: 4523–4533

    Google Scholar 

  5. Buckler-White AJ, Naeve CW, Murphy BR (1986) Characterisation of a gene encoding for M proteins which is involved in host range restriction of an avian influenza A virus in monkeys. J Virol 57: 697–700

    Google Scholar 

  6. Choppin PW, Compans RW, Scheild A, McSharry J, Lazarowitz S (1972) Structure and assembly of viral membranes. In: Fox FC (ed) Membrane research. Academic Press, New York, pp 163–179

    Google Scholar 

  7. Cox NJ, Kitame F, Kendal AP, Maassab HF, Naeve C (1988) Identification of sequence changes in the cold-adapted, live attenuated influenza vaccine strain, A/Ann/Arbor/6/60 (H2N2). Virology 167: 554–567

    Google Scholar 

  8. Desselberger U, Racaniello VR, Zazra JJ, Palese P (1980) The 3′ and 5′ end terminal sequences of influenza A, B and C virus RNA segments are highly conserved and show partial inverted complementarity. Gene 8: 315–328

    Google Scholar 

  9. Gregoriades A, Frangione B (1981) Insertion of influenza M protein into the viral lipid bilayer and localisation of site of insertion. J Virol 40: 323–328

    Google Scholar 

  10. Hankins RW, Nagata K, Bucher DJ, Popple S, Ishihama A (1989) Monoclonal antibody analysis of influenza virus matrix protein epitopes involved in transcription inhibition. Virus Genes 3: 111–126

    Google Scholar 

  11. Helenius, A (1992) Unpacking the incoming influenza virus. Cell 69: 577–578

    Google Scholar 

  12. Ito. T, Gorman OT, Kawaoka Y, Bean WJ, Webster RG (1991) Evolutionary analysis of the influenza A virus M gene with comparison of the M1 and M2 proteins. J Virol 65: 5491–5498

    Google Scholar 

  13. Klimov AI, Sokolov NI, Orlova NG, Ginzburg VP (1991) Correlation of amino acid residues in the M1 and M2 proteins of influenza virus with high yielding properties. Virus Res 19: 105–114

    Google Scholar 

  14. Klimov AI, Cox, NJ, Yotov WV, Rocha E, Alexandrova GI, Kendal AP (1992) Sequence changes in the live attenuated, cold-adapted variants of influenza A/Leningard/134/57 (H2N2). Virology 186: 795–797

    Google Scholar 

  15. Lamb RA, Lai C-J, Choppin PW (1981) Sequences of mRNA derived from genome RNA segment 7 of influenza: colinear and interrupted mRNAs code for overlapping proteins. Proc Natl Acad Sci USA 7: 1857–1861

    Google Scholar 

  16. Lentz MR, Air GM, Laver WG, Webster RG (1984) Sequence of the neuraminidase gene of influenza virus A/Tokyo/3/67 and previously uncharacterised monoclonal variants. Virology 135: 257–265

    Google Scholar 

  17. Markushin S, Ghiasi H, Sokolov N, Shilov A, Sinitsin B, Brown D, Klimov A, Nayak D (1988) Nucleotide sequence of RNA segment 7 and the predicted amino acid sequence of M1 and M2 proteins of FPV/Weybridge (H7N7) and WSN(H1N1) influenza A viruses. Virus Res 10: 263–272

    Google Scholar 

  18. Martin K, Helenius A (1991) Nuclear transport of influenza virus ribonucleoproteins, the viral matrix protein (M1) promotes export and inhibits import. Cell 67: 117–130

    Google Scholar 

  19. McCauley JW, Mahy BWJ, Inglis SC (1982) Nucleotide sequence of fowl plague virus RNA segment 7. J Gen Virol 58: 211–215

    Google Scholar 

  20. Murphy BR, Buckler-White AJ, London WT, Snyder MH (1989) Characterization of the M protein and nucleoprotein genes of an avian influenza virus which is involved in host range restriction in monkeys. Vaccine 7: 557–561

    Google Scholar 

  21. Nakajima, K, Nobusawa E, Nakajima S (1984) Genetic relatedness between A/Swine/Iowa 15/30 (H1N1) and human influenza viruses. Virology 139: 194–198

    Google Scholar 

  22. Ortin J, Martinez C, del Rio L, Davila M, Lopez-Galindez C, Villanueva N, Domingo E (1983) Evolution of the nucleotide sequence of influenza virus RNA segment 7 during drift of the H3N2 subtype. Gene 23: 233–239

    Google Scholar 

  23. Ruigrok RHH, Calder LJ, Wharton SA (1989) Electron microscopy of the influenza virus submembrane structure. Virology 173: 311–316

    Google Scholar 

  24. Samokhvalov EI, Karginov VA, Chizhikov VE, Blinov VM, Yuferov VP, Vasilenko SK, Uryvaev LV, Zhdanov VM (1985) Primary structure of RNA segment 7 of A/USSR/90/77 (H1N1) influenza virus. Bioorg Khim 11: 1080–1085

    Google Scholar 

  25. Schlesinger RW, Bradshaw GL, Barbone F, Reinacher M, Rott R, Husak P (1989) Role of haemagglutinin cleavage and expression of M1 protein in replication of A/WS/33, A/PR/8/34, and WSN influenza viruses in mouse brain. J Virol 63: 1695–1703

    Google Scholar 

  26. Scholtissek C, Vallbracht A, Flehmig B, Rott R (1979) Correlation of pathogenicity and gene constellation of influenza A viruses. II. Highly neurovirulent recombinants derived from non-neurovirulent or weakly neurovirulent parent virus strains. Virology 95: 492–500

    Google Scholar 

  27. Smeenk CA, Brown EG (1994) The influenza virus variant A/FM/1/47-MA possesses single amino acid replacements in the hemagglutinin, controlling virulence, and in the matrix protein, controlling virulence as well as growth. J Virol 68: 530–534

    Google Scholar 

  28. Sugiura A, Ueda M (1980) Neurovirulence of influenza virus in mice. I. Neurovirulence of recombinants between virulent and avirulent virus strains. Virology 101: 440–449

    Google Scholar 

  29. Sugrue RJ, Bahadur G, Zambon MC, Hall-Smith M, Douglas AR, Hay AJ (1990) Specific structural alteration of the influenza virus haemagglutinin by amantadine. EMBO J 9: 3469–3476

    Google Scholar 

  30. Ward AC, Harley VR (1994) Sequence analysis of influenza virus strain A/Shearwater/Australia/1/72 (in preparation)

  31. Winter G, Fields S (1980) Cloning of influenza cDNA into M13, the sequence of the RNA segment encoding the A/PR/8/34 matrix protein. Nucleic Acids Res 8: 1965–1974

    Google Scholar 

  32. Yasuda J, Toyoba T, Nakayama M, Ishihama A (1993) Regulatory effects of matrix protein variations on influenza virus growth. Arch Virol 133: 282–294

    Google Scholar 

  33. Ye Z, Baylor NW, Wagner RR (1989) Transcription-inhibition and RNA-binding domains of influenza A virus matrix protein mapped with antiidiotypic antibodies and synthetic peptides. J Virol 63: 3586–3594

    Google Scholar 

  34. Ye Z, Pal R, Fox JW, Wagner RR (1987) Functional and antigenic domains of the matrix (M1) protein of influenza A virus. J Virol 61: 239–246

    Google Scholar 

  35. Zebedee SL, Lamb RA (1989a) Growth restriction of influenza A virus by M2 protein antibody is genetically linked to the M1 protein. Proc Natl Acad Sci USA 86: 1061–1065

    Google Scholar 

  36. Zebedee SL, Lamb RA (1989b) Nucleotide sequence of influenza A virus RNA segment 7, a comparison of 5 isolates. Nucleic Acids Res 17: 2870

    Google Scholar 

  37. Zucker M, Stiegler P (1981) Optimal computer folding of large RNA sequences using thermodynamics and auxiliary information. Nucleic Acids Res 9: 133–148

    Google Scholar 

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  1. A. C. Ward

    Present address: Royal Melbourne Hospital, Department of Medicine, University of Melbourne, Parkville, Victoria, Australia

Authors and Affiliations

  1. Biomolecular Research Institute, Parkville, Australia

    A. C. Ward

  2. Russell Grimwade School of Biochemistry, University of Melbourne, Parkville, Victoria, Australia

    A. C. Ward

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Ward, A.C. Specific changes in the M1 protein during adaptation of influenza virus to mouse. Archives of Virology 140, 383–389 (1995). https://doi.org/10.1007/BF01309872

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  • DOI: https://doi.org/10.1007/BF01309872

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