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Molecular evolution of human paramyxoviruses

Nucleotide sequence analyses of the human parainfluenza type 1 virus NP and M protein genes and construction of phylogenetic trees for all the human paramyxoviruses

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Summary

The nucleotide sequences of the NP and M genes of human parainfluenza type 1 virus (HPIV-1) were determined. The NP gene was 1677 nucleotides long excluding polyadenylic acid. The NP gene contained a single large open reading frame (ORF), which encoded a polypeptide of 524 amino acids with a calculated molecular weight of 57,736. The M gene 1173 nucleotides long excluding the poly(A) tract and the sequence also contained a single large ORF which encoded a polypeptide of 348 amino acid with a molecular weight of 38,445, which was inconsistent with 28 kDa previously determined by SDS-PAGE. We aligned the deduced HPIV-1 NP and M protein sequences with 12 and 13 other paramyxoviruses, respectively, suggesting that a common tertiary structure was found in the NPs or Ms of HPIV-1, Sendai virus (SV), HPIV-3 and BPIV-3 and that other common structure was also maintained in these proteins of HPIV-2, SV 41 and 5, MuV, HPIV-4. Phylogenetic trees were constructed for the NP and M proteins of all the paramyxoviruses of which nucleotide sequences had been previously reported. Paramyxoviruses could be subdivided into two groups, i.e., PIV-1 group and PIV-2 group; the former group is composed of HPIV-1, SV, HPIV-3 and BPIV-3, and the latter group consists of HPIV-2, SV 41, SV 5, MuV, HPIV-4 A and HPIV-4 B.

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Authors and Affiliations

  1. Department of Microbiology, Mie University School of Medicine, Mie Prefecture, Japan

    K. Miyahara, S. Kitada, M. Yoshimoto, H. Matsumura, M. Kawano, H. Komada, M. Tsurudome, S. Kusagawa, M. Nishio & Y. Ito

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  1. K. Miyahara
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  2. S. Kitada
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  3. M. Yoshimoto
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  4. H. Matsumura
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  5. M. Kawano
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  6. H. Komada
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  7. M. Tsurudome
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  8. S. Kusagawa
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  9. M. Nishio
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Miyahara, K., Kitada, S., Yoshimoto, M. et al. Molecular evolution of human paramyxoviruses. Archives of Virology 124, 255–268 (1992). https://doi.org/10.1007/BF01309807

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  • DOI: https://doi.org/10.1007/BF01309807

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