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Three adjacent genes of African swine fever virus with similarity to essential poxvirus genes

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Summary

Nucleotide sequencing of the right end of theSalIj fragment of the highly virulent Malawi Lil 20/1 strain of African swine fever virus (ASFV) has revealed three adjacent genes with similarity to: serine-threonine protein kinases; members of the putative helicase superfamily SF2; and the vaccinia virus 56 kDa abortive late protein. All three genes are transcribed to the left with respect to the orientation of the ASFV genome. Gene L19IL predicts a protein similar to serine-threonine protein kinases including vaccinia virus gene B1R. Gene L19KL predicts a protein that is likely to be a nucleic acid-dependent ATPase, as it has similarity to both the poxvirus 70 kDa early transcription factor subunit and the poxvirus nucleoside triphosphatase I gene. Gene L19LL has extensive similarity to the vaccinia virus 56 kDa abortive late protein.

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References

  1. Aitken A (1990) Identification of protein consensus sequences: active site motifs, phosphorylation, and other post-translational modifications. Ellis Horwood, London

    Google Scholar 

  2. Almendral JM, Almazan F, Blasco R, Vinuela E (1990) Multigene families in African swine fever virus: family 110. J Virol 64: 2064–2072

    Google Scholar 

  3. Bankier AT, Weston KM, Barrell BG (1987) Random cloning and sequencing by the M 13/dideoxynucleotide chain termination method. Methods Enzymol 155: 51–93

    Google Scholar 

  4. Blasco R, Lopez-Otin C, Munoz M, Bockamp EO, Simon-Mateo C, Vinuela E (1990) Sequence and evolutionary relationships of African swine fever virus thymidine kinase. Virology 178: 301–304

    Google Scholar 

  5. Boursnell M, Shaw K, Yanez RJ, Vinuela E, Dixon LK (1991) The sequences of the ribonucleotide reductase genes from African swine fever virus show considerable homology with those of the orthopoxvirus, vaccinia virus. Virology 184: 411–416

    Google Scholar 

  6. Broyles SS, Fesler BS (1990) Vaccinia virus gene encoding a component of the viral early transcription factor. J Virol 64: 1523–1529

    Google Scholar 

  7. Broyles SS, Moss B (1987) Identification of the vaccinia virus gene encoding nucleotide triphosphate phosphohydrolase I, a DNA-dependent ATPase. J Virol 61: 1738–1742

    Google Scholar 

  8. Broyles SS, Moss B (1988) DNA-dependent ATPase activity associated with vaccinia virus early transcription factor. J Biol Chem 263: 10761–10765

    Google Scholar 

  9. Broyles SS, Yuen L, Shuman S, Moss B (1988) Purification of a factor required for transcription of vaccinia virus early genes. J Biol Chem 263: 10754–10760

    Google Scholar 

  10. Camacho A, Vinuela E (1991) Protein p 22 of African swine fever virus: an early structural protein that is incorporated into the membrane of infected cells. Virology 181: 251–257

    Google Scholar 

  11. Carrascosa AL, Sastre I, Vinuela E (1991) African swine fever virus attachment protein. J Virol 65: 2283–2289

    Google Scholar 

  12. Cohrs RJ, Condit RC, Pacha RF, Thompson CL, Sharma OK (1989) Modulation of ppp(A2′p)nA-dependent RNase by a temperature-sensitive mutant of vaccinia virus. J Virol 63: 948–951

    Google Scholar 

  13. Costa JV (1990) African swine fever virus. In: Darai G (ed) Molecular biology of iridoviruses. Kluwer Academic Publishers, Norwell, pp 247–270

    Google Scholar 

  14. Davison AJ, Moss B (1989 a) Structure of vaccinia virus early promoters. J Mol Biol 210: 749–769

    Google Scholar 

  15. Davison AJ, Moss B (1989 b) Structure of vaccinia virus late promoters. J Mol Biol 210: 771–784

    Google Scholar 

  16. de la Vega I, Vinuela E, Blasco R (1990) Genetic variation and multigene families in African swine fever virus. Virology 179: 234–246

    Google Scholar 

  17. Devereux J, Haeberli P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395

    Google Scholar 

  18. Dixon LK (1988) Molecular cloning and restriction enzyme mapping of an African swine fever virus isolate from Malawi. J Gen Virol 69: 1683–1694

    Google Scholar 

  19. Domen J, von Lindern M, Hermans A, Breuer M, Grosveld G, Berns A (1987) Comparison of the human and mouse pim-1 cDNAs: nucleotide sequence and immunological identification of the in vitro synthesized pim-1 protein. Oncogene Res 1: 103–112

    Google Scholar 

  20. Esparza I, Gonzalez JC, Vinuela E (1988) Effect of interferon-alpha, interferon-gamma and tumour necrosis factor on African swine fever virus replication in porcine monocytes and macrophages. J Gen Virol 69: 2973–2980

    Google Scholar 

  21. Gershon PD, Moss B (1990) Early transcription factor subunits are encoded by vaccinia virus late genes. Proc Natl Acad Sci USA 87: 4401–4405

    Google Scholar 

  22. Goebel SJ, Johnson GP, Perkus ME, Davis SW, Winslow JP, Paoletti E (1990) The complete DNA sequence of vaccinia virus. Virology 179: 247–266

    Google Scholar 

  23. Gonzalez A, Calvo V, Almazan F, Almendral JM, Ramirez JC, de la Vega I, Blasco R, Vinuela E (1990) Multigene families in African swine fever virus: family 360. J Virol 64: 2073–2081

    Google Scholar 

  24. Gorbalenya AE, Koonin EV, Donchenko AP, Blinov VM (1989) Two related super-families of putative helicases involved in replication, recombination, repair and expression of DNA and RNA genomes. Nucleic Acids Res 17: 4713–4730

    Google Scholar 

  25. Hammond JM, Dixon LK (1991) Vaccinia virus-mediated expression of African swine fever virus genes. Virology 181: 778–782

    Google Scholar 

  26. Hanks SK, Quinn AM (1991) Protein kinase catalytic domain sequence database: identification of conserved features of primary structure and classification of family members. Methods Enzymol 200: 38–62

    Google Scholar 

  27. Knighton DR, Zheng J, Ten Eyck LF, Ashford VA, Xuong NH, Taylor SS, Sowadski JM (1991 a) Crystal structure of the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253: 407–414

    Google Scholar 

  28. Knighton DR, Zheng J, Ten Eyck LF, Xuong NH, Taylor SS, Sowadski JM (1991 b) Structure of a peptide inhibitor bound to the catalytic subunit of cyclic adenosine monophosphate-dependent protein kinase. Science 253: 414–420

    Google Scholar 

  29. Kuznar J, Salas ML, Vinuela E (1981) Nucleoside triphosphate phosphohydrolase activities in African swine fever virus. Arch Virol 69: 307–310

    Google Scholar 

  30. Lipman DJ, Pearson WR (1985) Rapid and sensitive protein similarity searches. Science 227: 1435–1441

    Google Scholar 

  31. Lopez-Otin C, Freije JMP, Parra F, Mendez E, Vinuela E (1990) Mapping and sequence of the gene coding for protein p 72, the major capsid protein of African swine fever virus. Virology 175: 477–484

    Google Scholar 

  32. Lopez-Otin C, Simon C, Mendez E, Vinuela E (1988) Mapping and sequence of the gene encoding protein p 37, a major structural protein of African swine fever virus. Virus Genes 1: 291–303

    Google Scholar 

  33. Martin Hernandez AM, Tabares E (1991) Expression and characterization of the thymidine kinase gene of African swine fever virus. J Virol 65: 1046–1052

    Google Scholar 

  34. Matsushime H, Jinno A, Takagi N, Shibuya M (1990) A novel mammalian protein kinase gene (mak) is highly expressed in testicular germ cells at and after meiosis. Mol Cell Biol 10: 2261–2268

    Google Scholar 

  35. Moss B (1990) Poxviridae and their replication. In: Fields BN, Knipe DM (eds) Virology. Raven Press, New York, pp 2079–2111

    Google Scholar 

  36. Niles EG, Condit RC, Caro P, Davidson K, Matusick L, Seto J (1986) Nucleotide sequence and genetic map of the 16-kb vaccinia virusHindIII D fragment. Virology 153: 96–112

    Google Scholar 

  37. Pacha RF, Meis RJ, Condit RC (1990) Structure and expression of the vaccinia virus gene which prevents virus-induced breakdown of RNA. J Virol 64: 3853–3863

    Google Scholar 

  38. Paez E, Esteban M (1984 a) Resistance of vaccinia virus to interferon is related to an interference phenomenon between the virus and the interferon system. Virology 134: 12–28

    Google Scholar 

  39. Paez E, Esteban M (1984 b) Nature and mode of action of vaccinia virus products that block activation of the interferon-mediated ppp(A2'p)nA-synthetase. Virology 134: 29–39

    Google Scholar 

  40. Paez E, Garcia F, Fernandez CG (1990) Interferon cures cells lytically and persistently infected with African swine fever virus in vitro. Arch Virol 112: 115–127

    Google Scholar 

  41. Paoletti E, Moss B (1974) Two nucleic acid-dependent nucleoside triphosphate phosphohydrolases from vaccinia virus: nucleotide substrate and polynucleotide cofactor specificities. J Biol Chem 249: 3281–3286

    Google Scholar 

  42. Polatnick J, Pan IC, Gravell M (1974) Protein kinase activity in African swine fever virus. Arch Ges Virusforsch 44: 156–159

    Google Scholar 

  43. Rodriguez JF, Kahn JS, Esteban M (1986) Molecular cloning, encoding sequence, and expression of vaccinia virus nucleic acid-dependent nucleoside triphosphatase gene. Proc Natl Acad Sci USA 83: 9566–9570

    Google Scholar 

  44. Rodriguez JM, Salas ML, Vinuela E (1992) Genes homologous to ubiquitin-conjugating proteins and eukaryotic transcription factor SII in African swine fever virus. Virology 186: 40–52

    Google Scholar 

  45. Rohrmann G, Yuen L, Moss B (1986) Transcription of vaccinia virus early genes by enzymes isolated from vaccinia virions terminates downstream of a regulatory sequence. Cell 46: 1029–1035

    Google Scholar 

  46. Schwartz RM, Dayhoff MO (1979) Matrices for detecting distant relationships. In: Dayhoff MO (ed) Atlas of protein sequence and structure. National Biomedical Research Foundation, Washington DC, pp 353–358

    Google Scholar 

  47. Selten G, Cuypers HT, Boelens W, Robanus-Maandag E, Verbeek J, Domen J, van Beveren C, Berns A (1986) The primary structure of the putative oncogenepim-1 shows extensive homology with protein kinases. Cell 46: 603–611

    Google Scholar 

  48. Siegfried E, Perkins LA, Capaci TM, Perrimon N (1990) Putative protein kinase product of theDrosophila segment-polarity genezeste-white 3. Nature 345: 825–829

    Google Scholar 

  49. Staden R (1982) Automation of the computer handling of gel reading data produced by the shotgun method of DNA sequencing. Nucleic Acids Res 10: 4731–4751

    Google Scholar 

  50. Staden R (1990 a) Finding protein coding regions in genomic sequences. Methods Enzymol 183: 163–180

    Google Scholar 

  51. Staden R (1990 b) Searching for patterns in protein and nucleic acid sequences. Methods Enzymol 183: 193–211

    Google Scholar 

  52. Staden R, McLachlan AD (1982) Codon preference and its use in identifying protein coding regions in long DNA sequences. Nucleic Acids Res 10: 141–156

    Google Scholar 

  53. Strayer DS, Jerng HH, O'Connor K (1991) Sequence and analysis of a portion of the genomes of Shope fibroma virus and malignant rabbit fibroma virus that is important for viral replication in lymphocytes. Virology 185: 585–595

    Google Scholar 

  54. Tabares E, Martinez J, Martin E, Escribano JM (1983) Proteins specified by African swine fever virus: IV glycoproteins and phosphoproteins. Arch Virol 77: 167–180

    Google Scholar 

  55. Talavera A (1987) RNA polymerase-promoter recognition in African swine fever and vaccinia viruses. In: African swine fever and pig immunology. Proceedings of meeting, Salamanca 1987

  56. Tartaglia J, Winslow J, Goebel S, Johnson GP, Taylor J, Paoletti E (1990) Nucleotide sequence analysis of a 10.5 kbpHind III fragment of fowlpox virus: relatedness to the central portion of the vaccinia virusHind III D region. J Gen Virol 71: 1517–1524

    Google Scholar 

  57. Tommasino M, Ricci S, Galeotti CL (1988) Genome organization of the killer plasmid pGK 12 fromKluyveromyces lactis. Nucleic Acids Res 16: 5863–5878

    Google Scholar 

  58. Traktman P, Anderson MK, Rempel RE (1989) Vaccinia virus encodes an essential gene with strong homology to protein kinases. J Biol Chem 264: 21458–21461

    Google Scholar 

  59. Vinuela E (1985) African swine fever virus. Curr Top Microbiol Immunol 116: 151–170

    Google Scholar 

  60. Wilkinson PJ (1989) African swine fever virus. In: Pensaert MB (ed) Virus infections of porcines. Elsevier, Amsterdam, pp 17–35

    Google Scholar 

  61. Woodgett JR (1990) Molecular cloning and expression of glycogen synthase kinase-3/factor A. EMBO J 9: 2431–2438

    Google Scholar 

  62. Yuen L, Moss B (1987) Oligonucleotide sequence signaling transcriptional termination of vaccinia virus early genes. Proc Natl Acad Sci USA 84: 6417–6421

    Google Scholar 

  63. Zakut-Houri R, Hazum S, Givol D, Telerman A (1987) The cDNA sequence and gene analysis of the humanpim oncogene. Gene 54: 105–111

    Google Scholar 

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  1. Plum Island Animal Disease Center, USDA, ARS, NAA, Greenport, New York, USA

    P. C. Roberts, Z. Lu, G. F. Kutish & D. L. Rock

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  1. P. C. Roberts
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  2. Z. Lu
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  3. G. F. Kutish
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Roberts, P.C., Lu, Z., Kutish, G.F. et al. Three adjacent genes of African swine fever virus with similarity to essential poxvirus genes. Archives of Virology 132, 331–342 (1993). https://doi.org/10.1007/BF01309543

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  • DOI: https://doi.org/10.1007/BF01309543

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