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Effects of 16, 16-dimethyl prostaglandin E2 on alkaline secretion in isolated canine gastric mucosa

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Abstract

An isolated fundic mucosal preparation of dog stomach which is capable of exhibiting an alkaline secretion is described. A stable secretion was established 40 min to 1 hr after the mucosa was pretreated with the H2-antagonist cimetidine to block spontaneous acid output. Alkaline secretion decreased when Ca2+ was removed from the nutrient solution. This secretion was stimulated by dibutyryl cyclic GMP, but was not altered by acetylcholine, carbachol, or 16,16-dimethyl PGE2. Alkaline secretion from a similar antral mucosal preparation was stimulated by 16,16-dimethyl PGE2. We conclude that the 16,16-dimethyl PGE2-stimulated bicarbonate secretion previously demonstrated inin vivo canine fundic mucosa is not the result of a direct effect of PG on gastric mucosal cells and that an intact blood circulation or cholinergic innervation is required for this action to occur.

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Authors and Affiliations

  1. Department of Physiology and Cell Biology, University of Texas Medical School at Houston, 77030, Houston, Texas

    Yuh-Jyh Kuo MS, Linda L. Shanbour PhD &  Thomas A. Miller MD

  2. Department of Surgery, The University of Texas, Medical School at Houston, 6431 Fannin, Room 4266, 77030, Houston, Texas

    Yuh-Jyh Kuo MS, Linda L. Shanbour PhD &  Thomas A. Miller MD

Authors
  1. Yuh-Jyh Kuo MS
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  2. Linda L. Shanbour PhD
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  3. Thomas A. Miller MD
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Additional information

This study was supported by research grants AM 25838 and AA 00194 from the National Institutes of Health.

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Kuo, YJ., Shanbour, L.L. & Miller, T.A. Effects of 16, 16-dimethyl prostaglandin E2 on alkaline secretion in isolated canine gastric mucosa. Digest Dis Sci 28, 1121–1126 (1983). https://doi.org/10.1007/BF01295812

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  • DOI: https://doi.org/10.1007/BF01295812

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