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The vacuole system in stomatal cells ofAllium. vacuole movements and changes in morphology in differentiating cells as revealed by epifluorescence, video and electron microscopy

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Summary

The development of autofluorescent vacuoles in the stomatal cells ofAllium cepa andA. vineale was investigated using fluorescence microscopy of live cells, low light level television, cytochemistry and electron microscopy. During cell differentiation, the vacuole undergoes two major changes in morphology. In an intermediate form, it consists of a reticulum or network of interlinked tubules and small chambers. The network is formed from globular cisternae in very young GMCs and is maintained as a reticulum until it is transformed back into a globular form later in the differentiation of guard cells. The network thus remains intact through the course of one cell division. During its existence, the reticulum undergoes complex movements and rearrangements. The significance of these changes in the vacuole is discussed in terms of vacuole ontogeny and function and the mechanisms that control motility in plant cells.

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Abbreviations

DIC:

differential interference contrast

TV:

television

L3TV:

low light level television

GMC:

guard mother cell

I2V:

double image intensifier vidicon

I3V:

triple image intensifier vidicon

CCU:

camera control unit

SEG:

special effects generator

VTR:

video tape recorder

VCR:

video cassette recorder

TDG:

time-date generator

TBC:

time base corrector

SYNC:

synchronization

BL:

blanking

VD:

vertical drive

HD:

horizontal drive

PMT:

photomultiplier tube

ALC:

automatic light compensation

LED:

light emitting diode.

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Authors and Affiliations

  1. Department of Botany, University of Georgia, 30602, Athens, GA, USA

    B. A. Palevitz, D. J. O'Kane, R. E. Kobres & N. V. Raikhel

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  1. B. A. Palevitz
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  2. D. J. O'Kane
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  3. R. E. Kobres
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  4. N. V. Raikhel
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Palevitz, B.A., O'Kane, D.J., Kobres, R.E. et al. The vacuole system in stomatal cells ofAllium. vacuole movements and changes in morphology in differentiating cells as revealed by epifluorescence, video and electron microscopy. Protoplasma 109, 23–55 (1981). https://doi.org/10.1007/BF01287629

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  • DOI: https://doi.org/10.1007/BF01287629

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