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Calcium-induced fusion of fusogenic wild carrot protoplasts

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Summary

Rather than selecting for a chemical fusogen one can select for a fusogenic plant membrane (i.e., one that will fuse readily). Wild carrot suspension culture cells can be grown under conditions which cause the released protoplasts to have a high potential to fuse. Protoplast fusion is enhanced by calcium and inhibited by EGTA. When 10mM calcium (pH6.0) is added, fusion percentages of 60% are common. The mild fusion treatment appears to have no effect on callus regeneration and differentiation.

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Authors and Affiliations

  1. Botany Department, North Carolina State University, 27650, Raleigh, NC, USA

    W. F. Boss, H. D. Grimes & A. Brightman

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  1. W. F. Boss
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  2. H. D. Grimes
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  3. A. Brightman
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Boss, W.F., Grimes, H.D. & Brightman, A. Calcium-induced fusion of fusogenic wild carrot protoplasts. Protoplasma 120, 209–215 (1984). https://doi.org/10.1007/BF01282601

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  • DOI: https://doi.org/10.1007/BF01282601

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