Summary
The legume seed reserve protein vicilin has been localized in developing pea cotyledons by immunogold labelling on sections of glutaraldehyde-osmium-fixed, resin-embedded tissue. By treating sections with periodate and acid before antibody labelling, a 20-fold increase in specific antibody binding was observed. The densest label occurred over vacuolar contents, previously identified as the site of protein storage, and over electron-dense cisternae occasionally seen to be continuous with the vacuolar contents. Vicilin was also associated with the rough endoplasmic reticulum, the implied site of synthesis, and in electron-dense Golgi vesicles which, we suggest, are a vehicle by which newly synthesized protein is relocated into the vacuole.
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Craig, S., Goodchild, D.J. Periodate-acid treatment of sections permits on-grid immunogold localization of pea seed vicilin in ER and Golgi. Protoplasma 122, 35–44 (1984). https://doi.org/10.1007/BF01279435
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DOI: https://doi.org/10.1007/BF01279435