Skip to main content
Log in

Thymidine-3H labelling of mycoplasma-like bodies in roots of rice plants affected by a yellows-type disease

  • Published:
Protoplasma Aims and scope Submit manuscript

Summary

Mycoplasma-like bodies have been described in the parenchyma and companion cells of the phloem in roots of rice (Oryza sativa) plants affected by yellows-type disease. Electron microscopy autoradiographic study has shown that these forms of mycoplasmalike bodies, rich in ribosomes, incorporate thymidine-3H. This fact suggests that these organisms are mycoplasma and are capable of active multiplication.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Similar content being viewed by others

References

  • Amici, A., R. Ballestros, J. A. Batalla eG. Belli, 1970: Corpi riferibili a micoplasmi in piante di riso spagnolo affetto da “enrochat”. Riv. Pat. Veg. S. IV5, 247–253.

    Google Scholar 

  • Baldacci, E., A. Amici, G. Belli eG. Corbetta, 1970: Il giallume del riso: osservazioni e ricerche su sintomatologia, epidemiologia ed ezimologia. Il Riso19, 3–9.

    Google Scholar 

  • Belli, G., 1969: Mycoplasma-like particles in clarified extracts of diseased rice plants. Riv. Pat. Veg. S. IV5, 3–11.

    Google Scholar 

  • Caro, L. G., andR. P. van Tubergen, 1962: High-resolution autoradiography. I. Methods. J. Cell Biol.15, 173–188.

    Google Scholar 

  • Cousin, M. T., H. Darpoux, A. Faivre-Amiot etT. Staron, 1970: Sur la presence de micro-organismes de type mycoplasme dans le parenchyme cortical de feverolles presentant des symptomes de virescence. C. R. Acad. Sci. (Paris)271, 1182–1184.

    Google Scholar 

  • Favali, M. A., andG. Lombardo, 1970: Thymidine-3H labelling of mycoplasma in sieve cells. Ann. Microbiol.20, 81–83.

    Google Scholar 

  • Galvez, E., eE. Shikata, 1969: Microorganismos similares a los micoplasmas o al grupo PLT (Psittacosis-lymphogranuloma-trachoma), probables agentes causales del enanismo amarillo del Arroz. Agricoltura trop.25 (2), 109–115.

    Google Scholar 

  • Kopriwa, B. M., 1967: The influence of development on the number and appearance of silver grains in electron microscopic radioautography. J. Histochem. Cytochem.15, 501–515.

    Google Scholar 

  • Lafléche, D., etJ. M. Bové, 1970: Structures de type mycoplasme dans les feuilles d'orangers atteintes de la maladie du “Greening”. C. R. Acad. Sci. (Paris)270, 1915–1917.

    Google Scholar 

  • Lombardo, G., M. Bassi, andF. M. Gerola, 1970: Mycoplasma development and cell alterations in white clover affected by clover dwarf. An electron microscopy study. Protoplasma70, 61–71.

    Google Scholar 

  • Luft, J. H., 1961: Improvement in epoxy resin embedding methods. J. biophys. biochem. Cytol.9, 409–414.

    Google Scholar 

  • Maramorosch, K., E. Shikata, andR. R. Granados, 1968: Structures resembling mycoplasma in diseased plants and in insect vectors. Trans. N. Y. Acad. Sci. Series II30, 841–855.

    Google Scholar 

  • Pellegrini, S., G. Belli, andF. M. Gerola, 1969: Mycoplasma-like bodies in rice plants infected with a yellows-type disease. Giorn. Bot. Ital.103, 395–399.

    Google Scholar 

  • —, andF. M. Gerola, 1970: Mycoplasma-like bodies in phloem cells ofOryza sativa. Electron microscope observations. Caryologia23, 639–646.

    Google Scholar 

  • Shikata, E., K. Maramorosch, andK. C. Ling, 1969: Presumptive mycoplasma etiology of yellows diseases. FAO Plant Protection Bulletin17, 121–128.

    Google Scholar 

  • —, andT. Matsumoto, 1968: Mycoplasma-like structures in plants diseased with American aster yellows, corn stunt, Philippine rice yellow dwarf, and Taiwan sugar cane white leaf. Ann. Phytopathol. Soc. (Japan)34, 208–209.

    Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Additional information

This investigation was supported by a grant of C.N.R., Roma.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Amici, A., Favali, M.A. Thymidine-3H labelling of mycoplasma-like bodies in roots of rice plants affected by a yellows-type disease. Protoplasma 75, 37–43 (1972). https://doi.org/10.1007/BF01279394

Download citation

  • Received:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF01279394

Keywords

Navigation