Summary
A RAPD marker specific to the dwarf off-type (hereafter known as dwarf) from micropropagation of Cavendish banana (Musa spp. AAA) cultivars New Guinea Cavendish and Williams was identified following an analysis of 57 normal (true-to-type) and 59 dwarf plants generated from several different micropropagation events. Sixty-six random decamer primers were used in the initial screen, of which 19 (28.8%) revealed polymorphisms between normal and dwarf plants. Primer OPJ-04 (5'-CCGAACACGG-3') was found to amplify an approx. 1.5 kb band which was consistently present in all normal but absent in all dwarf plants of both cultivars. Reliable detection of dwarf plants was achieved using this marker, providing the only available means ofin vitro detection of dwarfs. The use of this marker could facilitate early detection and elimination of dwarfs from batches of micropropagated bananas, and may be a useful tool in determining what factors in the tissue culture process lead to this off type production.Other micropropagation-induced RAPD polymorphisms were observed but were not associated with the dwarf trait.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Connolly AG, Godwin ID, Cooper M, DeLacy IH (1994) Theor Appl Genet 88:332–336
Damasco OP, Smith MK, Godwin ID Adkins SW (1996) Aust J Expt Agric 36(2) (in press)
Daniells J, Smith MK (1991) ACIAR Technical Reports 14/9, Australia
Graham GC, Mayers P, Henry RJ (1994) BioTechniques 16:2–3
Howell EC, Newbury HJ, Swennen RL, Withers LA, Ford-Lloyd BV (1994) Genome 37:328–332
Hwang SC, Ko WH (1987)In Persley GJ, De Langhe EA (eds) Banana and Plantain Breeding Strategies. ACIAR Proceedings No.21, pp 151-56
Israeli Y, Reuveni O, Lahav E (1991) Sci Hortic 48:71–88
Kaemmer D, Afza R, Weising K, Kahl G, Novak FJ (1992) Bio/T'echnology 10:1030–1035
Miklas PN, Stavely JR, Kelly JD (1993) Theor Apps Genet 85:745–749
Penner GA, Chong J, Levesque-Lemay M, Molnar SJ, Fedak G (1993) Theor Appl Genet 85:702–705
Rani V, Parida A, Raina SN (1995) Plant Cell Reports 14:459–462
Reuveni O, Israeli Y, Degan H, Eshdat Y (1986) Research Report (1986) International Board of Plant Genetic Resources, Rome
Reuveni O (1990) In Jarret RL (ed) Identification of Genetic Diversity in the GenusMusa INIBAP, Montpellier, France, pp 108–113
Scowcroft WR (1984) IPBGR Report 184/152, Rome
Smith MK (1988) Fruits 43:219–223
Smith MK, Drew RA (1990) Aust J Expt Agric 30:575–578
Smith MK, Hamill SD (1993) Aust J Expt Agric 33:639–644
Stiles JI, Lemme C, Sondur S, Morshidi MB, Manshardt R (1993) Theor App] Genet 85:697–701
Stover RE (1987)In Persley GJ, De Langhe EA (eds) Banana and Plantain Breeding Strategies. ACIAR Proceedings No.21, pp 151-56
Taylor PWJ, Geijskes JR, Ko HL, Fraser TA, Henry RJ, Birch RG (1995) Theor Appl Genet 90:1169–1173
Williams JG, Kubelik AR, Livak KJ, Rafalski JA, Tingey SV (1990) Nucleic Acid Res 18:6531–6535
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Damasco, O.P., Graham, G.C., Henry, R.J. et al. Random amplified polymorphic DNA (RAPD) detection of dwarf off-types in micropropagated Cavendish (Musa spp. AAA) bananas. Plant Cell Reports 16, 118–123 (1996). https://doi.org/10.1007/BF01275464
Received:
Revised:
Issue Date:
DOI: https://doi.org/10.1007/BF01275464