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Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma

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Summary

Although the existence of three different cDNA forms of human GTP cyclohydrolase I (GCH I) have been reported (Togari et al., 1992), the full-length sequence of any human GCH I cDNA involving poly (A) tail has not yet been documented. In the present study, we first isolated a full-length cDNA clone encoding human GCH I type 1 from human pheochromocytoma cDNA library. The length of the cDNA insert was 2,921 base pairs including poly (A) tail. RNA blot analysis showed a single niRNA species of 4.0kb in human pheochromocytoma tissue.

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Author notes

  1. T. Nagatsu

    Present address: Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

Authors and Affiliations

  1. Department of Pharmacology, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

    T. Nomura, M. Ohtsuki, S. Matsui, C. Sumi-Ichinose, H. Nomura & Y. Hagino

  2. Department of Surgery, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

    K. Iwase

  3. Institute for Comprehensive Medical Science, Fujita Health University School of Medicine, Toyoake, Aichi, Japan

    H. Ichinose & K. Fujita

Authors
  1. T. Nomura
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  2. M. Ohtsuki
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  3. S. Matsui
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  4. C. Sumi-Ichinose
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  5. H. Nomura
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  6. Y. Hagino
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  7. K. Iwase
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  8. H. Ichinose
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  9. K. Fujita
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  10. T. Nagatsu
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Additional information

The cDNA sequence data reported in this paper have been submitted to the EMBL/ GenBank Data Libraries under the accession number U19523

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Nomura, T., Ohtsuki, M., Matsui, S. et al. Isolation of a full-length cDNA clone for human GTP cyclohydrolase I type 1 from pheochromocytoma. J. Neural Transmission 101, 237–242 (1995). https://doi.org/10.1007/BF01271561

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  • DOI: https://doi.org/10.1007/BF01271561

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