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Transforming growth factor β1 inhibits IL-3- and IL-4-dependent mouse connective tissue-type mast cell proliferation

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Abstract

Transforming growth factor Β1 (TGFΒ1) is a regulator of cell proliferation and differentiation. Using a mouse peritoneal cell-derived mast cell culture system, we investigated the effects of TGFΒ1 on mast cell proliferation. TGFΒ1 inhibited IL-3- and IL-4-dependent connective tissue-type mast cell proliferation. The effect was concentration dependent: 50% inhibition was observed with 1.0 ng/ml TGFΒ1 and the maximal inhibitory effect (no proliferation), was observed with 10 ng/ml. Flow cytometric analysis suggested that the inhibitory effect of TGFΒ1 was due to blocking of both G1 and G2 phases. Both control and TGFΒ1-treated mast cells showed similar histamine release induced by the calcium ionophore, A23187. TGFΒ1 seems to be an important negative regulator of connective tissue-type mast cell proliferation with apparently no appreciable effect on mast cell function.

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Authors and Affiliations

  1. Department of Dermatology, Asahikawa Medical College, 4-5-3-11, Nisikagura, Asahikawa, Hokkaido, Japan

    Noriaki Toyota, Yoshio Hashimoto, Shinobu Matsuo & Hajime Iizuka

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  1. Noriaki Toyota
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  2. Yoshio Hashimoto
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  3. Shinobu Matsuo
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  4. Hajime Iizuka
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Toyota, N., Hashimoto, Y., Matsuo, S. et al. Transforming growth factor β1 inhibits IL-3- and IL-4-dependent mouse connective tissue-type mast cell proliferation. Arch Dermatol Res 287, 198–201 (1995). https://doi.org/10.1007/BF01262332

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  • DOI: https://doi.org/10.1007/BF01262332

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